8. Using recombinant DNA techniques (which willbe described in Chapter 9), it is possible to take theDNA of a gene from any source and place it on achromosome in the nucleus of a yeast cell. Whenyou take the DNA for a human gene and put it into ayeast cell chromosome, the altered yeast cell canmake the human protein. But when you remove theDNA for a gene normally present on yeast mitochondrial chromosomes and put it on a yeast chromosome inthe nucleus, the yeast cell cannot synthesize the correctprotein, even though the gene comes from the sameorganism. Explain. What would you need to do to ensurethat such a yeast cell could make the correct protein?

7) A patient has discovered a lump in their breast. The doctors decide to biopsy (take tissue samples from) the lump. They take 7 tissue samples from different regions of the lump. Would you expect the genetic profile/mutations in every single sample of cells to be the same? Why or why not?

1. The simplest mobile elements in bacteria are called ___________________.A. insertion sequencesB. genesC. transposonsD. transposasesE. none of the above 2. ______________ worked in the area of chromosome biology using maize and suggested segments of the chromosome could "jump" to another location.A. Barbara HersheyB. Martha ChaseC. Barbara McClintockD. Rosalind FranklinE. None of the above 3. Transposition is a process in which a discrete DNA entity can move between DNA sites that lack homology using a self-encoded protein called a _______________.A. recombinaseB.kinaseC. transposaseD. mobilaseE. none of the above

6. Suppose a particular gene is required for early development and also later for development of a particulartissue, such as the adult nervous system. By generating a homozygous mutant clone in that tissue of a heterozygote, researchers can circumvent the lethalitythat would result if the entire animal is homozygousfor a loss-of-function mutation in that gene.A technique called MARCM (Mosaic Analysiswith a Repressible Cell Marker) was developed to enable Drosophila geneticists to generate homozygousmutant cell clones that are marked by the presence of areporter protein such as GFP. Marker expression enables the investigator to observe clearly the mutantphenotype within a clone of mutant cells. This technique relies on a yeast protein called Gal80 that is anegative regulator of the Gal4 protein described previously in Solved Problem II. Gal80 binds to Gal4 andprevents it from activating transcription. The idea ofMARCM is that Gal4/UASG-driven GFP expression isblocked by Gal80 throughout…