24. In recombinant DNA technology, genes are usually inserted into what form of DNA before being transferred into bacteria? - [ ] Teddy bear - [ ] Nucleus - [ ] Transposon - [ ] Ribosome - [ ] Plasmid 25. In PCR , what is happening at the denaturation step (94 )? - [ ] RNA polymerase creates a new RNA strand . - [ ] DNA strands come apart . - [ ] DNA polymerase creates a new DNA strand. - [ ] Primers bind to the target DNA. - [ ] Nucleotides bind to the primer .

24. In recombinant DNA technology, genes are usually inserted into what form of DNA before being transferred into bacteria? - [ ] Teddy bear - [ ] Nucleus - [ ] Transposon - [ ] Ribosome - [ ] Plasmid 25. In PCR , what is happening at the denaturation step (94 )? - [ ] RNA polymerase creates a new RNA strand . - [ ] DNA strands come apart . - [ ] DNA polymerase creates a new DNA strand. - [ ] Primers bind to the target DNA. - [ ] Nucleotides bind to the primer .

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter15: Genomes And Genomics

Section: Chapter Questions

Problem 6QP: Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel,...

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To amplify a section of DNA using the polymerase chain reaction (PCR), all you need to load into the tube is 1) a buffer solution, 2) the DNA you want to amplify, 3) some DNA nucleotides, 4) a polymerase (like Taq polymerase), and O an RNA polymerase a set of forward and reverse primers some phospholipids for a cell membrane some ribosomes
16. Which of the following enzyme repairs the DNA backbone during molecular cloning (that is you are inserting the DNA fragment into a plasmid and the bases in the sticky ends are aligned but the backbone needs to be repaired). a) reverse transcriptase b) restriction enzymes c) DNA ligase d) polymerase
The figure at the right shows a partially drawn replication fork. a) Annotate this figure to show the proper location of each of the following: • template DNA strand polarity • DNA polymerase I Topoisomerase (gyrase) • leading strand daughter fragment(s) lagging strand daughter fragment(s) Single stranded binding proteins • DNA polymerase III • Primase • Ligase • Helicase
Recombinant DNA molecules are produced by incubating plasmids that have been broken open by a restriction enzyme together with DNA molecules containing complementary sticky ends in the presence of the enzyme ? 1) exonuclease 2) DNA connectase O 3) DNA ligase 4) DNA polymerase O 5) endonuclease
Match the following (most appropriate combinations): helicase Topoisomerase DNA Polymerase III DNA Polymerase I Primase [Choose ] Replicates bacterial chromosomes Separates DNA strands Changes Supercoiling Has a 5-to-3 exonuclease activity RNA synthesis Changes Supercoiling Has a 5-to-3 exonuclease ac RNA synthesis
36. Which is the sequence of steps used to amplify DNA with PCR during a single cycle? 1) Primers bind (anneal) to complementary sequences of the target DNA. 2) DNA is heated to a high temperature to cause denaturation (DNA is single-stranded). 3) DNA polymerase extends the 3’OH of primers to make copies of the target DNA. A) 1, 2 and 3 B) 3, 2, and 1 C) 2, 1, and 3 D) 1, 3, and 2
1) Follow the steps involved in DNA or gene cloning 2) In what process do bacteria take up the recombinant plasmid DNA 3) What are the advantages or replications of gene cloning?
Considering the Polymerase Chain Reaction (PCR), we can state that: a) It is a technique that promotes linear amplification of the number of DNA molecules in a sample. b) Depends on an initial template which can be a DNA or RNA molecule c) The reaction must contain, in addition to the template sequence, nitrogenous bases, sense and antisense primers and DNA polymerase enzyme. d)The primer sense rings on the template strand 3’>5’, extending from the 3’ end e)
Below is a list of "solutions" to problems encountered when bacteria replicate DNA. Match the "solution" to the specific "problem" encountered during DNA replication ligase [Choose ] [Choose ] helicase using RNA as template to make DNA conversion of ds DNA into ss templates Primase synthesis of most of DNA replacement of RNA with DNA DNA Polymerase I relief of chromosome supercoiling synthesis of short RNA primer protection of ss DNA templates DNA Polymerase III covalent linkage of Okazaki fragments Bacterial Gyrase [ Choose ] SSBP's [ Choose ]
Which is not a property of DNA polymerase? a) It requires a primer to begin synthesis b) It adds dNTPS only in a 5' to 3' direction c) It opens the two strands of template DNA at the repliction fork d) Its exonuclease activity is used in proofreading. 6. In an analysis of the composition of double-stranded DNA, which will always be found? a) A = G b) G=T c) A+T= G+ C d) A+ G C+T e) A = C 7._ DNA is synthesized through a process that is a) conservative b) semi-discontinuous c) bi-directional d) both a and b e) both b and c 8. The role of DNA ligase is to a) stabilize unwound DNA b) join Okazaki fragments together c) synthesize the RNA primer d) unwind the double helix e) hold the polymerase-for-extension in place at the replication fork 9.
11. Which of the following enzymes remove supercoiling in replicating DNA ahead of the replication fork? a) Topoisomerases b) Primases c) Helicases d) DNA polymerases
With respect to DNA and RNA polymerases which statement is correct? A) Only DNA polymerases are DNA template driven. B) Only DNA polymerases require primers. OC) Only RNA polymerases are DNA template driven. OD) Only RNA polymerases require primers. E) Both require a DNA template, activated monomer units and a primer