7) CRSPR-cas9 has revolutionized are ability to edit genomes. How hasthe CRSPR-cas9 system increased the efficiency of introducing mutations to mammalian genomes?
Q: Does one have a practical idea of the role of CRISPR/Cas9 in genome editing via cutting of DNA?
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Q: 7) CRSPR-cas9 has revolutionized are ability to edit genomes. How has the CRSPR-cas9 system…
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7) CRSPR-cas9 has revolutionized are ability to edit genomes. How hasthe CRSPR-cas9 system increased the efficiency of introducing mutations to mammalian genomes?
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- 7) CRSPR-cas9 has revolutionized are ability to edit genomes. How has the CRSPR-cas9 system increased the efficiency of introducing mutations to mammalian genomes?i) Suppose we want to insert the GFP sequence after the promoter of a gene X to create a fusion protein. Please describe the strategy that you will follow to edit this gene with CRISPR-Cas9 technology, in order to visualize the expression of gene X in live cells. (ii) Please explain how a knockout mouse for a gene X can be produced with CRISPR-Cas9 technologyThe ability to selectively modify the genome in the mouse has revolutionized mouse genetics. Outline the procedure for generating a knockout mouse at a specific genetic locus. How can the loxP-Cre system be used to conditionally knock out a gene? What is an important medical application of knockout mice?
- In the regulatory switch experiment, what do you predict is a likely outcome in mice with the bat-mouse construct? Select one 1.) change in the nucleotide sequence of the Prx1 transcribed region 2.) change in the amount of Prx1 mRNA produced 3.)change in the amino acid sequence of the PRX1 protein 4.)change in the nucleotide sequence of the Prx1 mRNA producedMy Avya mouse is brownish because a) the CpG island with the transposon is unmethylated. b) it was feed an unsupplemented diet. c) transposon has enabled agouti gene expression. d) none of the above.What are site-recombinases? Describe in detail how cre- recombinase can be used to decipher the roles of specific genes and proteins in complex multicellular organisms? Explain how the cre-lox system can be used to examine the role of a particular gene in a specific type of tissue?
- Q1: What common mechanism is employed by the guide RNA to find its target DNA sequence? Q2: How many strands of DNA must Cas9 cut to be effective? Q3: Does Cas9 also cause the deletion of DNA from the genome?CRISPR-Cas9 was first developed as a molecular tool in 2012; during the next few years, its use in molecular biology exploded, as scientists around the world began applying it to many different research problems, and hundreds of research papers describing its application were published. Explain why CRISPR-Cas is such a powerful tool in molecular genetics.How might our knowledge about telomeres and telemerase be applied to anti-aging strategies? Are such therapies or strategies being developed?
- Propose some specific uses of a modified CRISPR-Cas system as a general RNA-guided device for altering cellular functions. What might these functions be, and how could CRISPR-Cas be used to study them?Most organisms display a circadian rhythm, a cycling of biological processes that is roughly synchronized with day length. In Drosophila, pupae eclose (emerge as adults after metamorphosis) at dawn. a)Using this knowledge how would screen for Drosophila mutants that have an impaired circadian rhythm? b)In each case, how would you clone the genes you identified by mutation?Given these options, which best describes the Cre-Lox system A) can be applied to control the expression of any gene desired by researchers. B) can only be used to control expression of genes encoding fluorescent proteins. C) is interesting in theory, but cannot be applied practically in research D) Only allows us to create glowing mice for the pet industry.