CRISPR-Cas9 was first developed as a molecular tool in 2012; during the next few years, its use in molecular biology exploded, as scientists around the world began applying it to many different research problems, and hundreds of research papers describing its application were published. Explain why CRISPR-Cas is such a powerful tool in molecular genetics.

Q: Early gene-cloning experiments involved insertion at one restriction site in the vector; for…

A: Answer: Introduction: Restriction endonucleases enzymes which cut DNA at precise recognition sites.…

Q: Does one have a practical idea of the role of CRISPR/Cas9 in genome editing via cutting of DNA?

A: A molecular process in which some discontinuous change to nucleotide sequences in their genomic DNA…

Q: Replication is the first process that should occur in the Central Dogma of Molecular Genetics. Why?…

A: Introduction: The central dogma of molecular genetics states that DNA is synthesized from a DNA…

Q: please list all possible products in a sequencing reaction using ddGTPA as a terminator based on the…

A: Ans: Chain termination sequencing: It is sanger sequencing method developed by Frederick sanger. The…

Q: What is the difference between nonhomologous end-joining (NHEJ) and homology-directed repair (HDR)…

A: Genome editing can be defined as the way of of making  the specific changes to the DNA of a cell or…

Q: Like many biotechnologies, CRISPR/Cas9 was originally discovered as system that naturally occurs in…

A: Biotechnology is the branch of biology which deals with the utilisation of cells and biomolecular…

Q: Although many cloning applications involve introducing recombinant DNA into bacterial host cells,…

A: Cloning can be defined as the process by which identical copies of the organisms are created. More…

Q: Does SARS-CoV-2 conform to the central dogma of molecular biology that was coined by Francis Crick?…

A: Introduction: SARS-Cov-2 is a member of a large family of viruses called coronavirus disease 19…

Q: Under most practical circumstances, Crispr-cas9 technology can edit a eukaryote's genome (to replace…

A: Correct response to this particular question would be, 80%.

Q: How the amplification of other genomic methods such as optical mapping helps to overcome the…

A: The amplification of other genomic methods like optical mapping  has definitely improved the…

Q: Now that you understand how the CRISPR-Cas9 system works, think back to the experiments discussed in…

A: CRISPR-Cas9 is a genome editing tool. It helps researchers to edit or change the gene from the…

Q: Forty years after its development, the use of recombinant DNA technology is widespread and is found…

A: The technique that deals with the incorporation of a foreign or desired DNA fragment into another…

Q: Draw roughly the comparative electrophoretic mobilities of close circular DNA, open circular DNA and…

A: Since there are multiple question asked in this particular question, I will answer the first one for…

Q: What does CRISPR-Cas stand for and how does it work? What are the molecules involved with CRISPR-Cas

A: Introduction Genome editing:- It is also known as gene editing or genome engineering. It is a type…

Q: You have isolated a transposable element from the human genome and have determined its DNA…

A: Transposable element also known as jumping gene, is a DNA sequence which can change its position in…

Q: Describe one effect of a Cas (CRISPR-Associated) protein on DNA molecules.

A: The (CRISPR) Cas system acts in a sequence-specific manner by recognizing and cleaning foreign DNA…

Q: What is Central Dogma of Molecular Biology?

A: The organisms that contain DNA as a genetic material shows a fascinating process within the cell is…

Q: What is CRISPR-Cas9? What is one advantage and one disadvantage of this technology?

A: Gene editing is a technology in which Genetic material of an organism is altered and remodelled.…

Q: Does SARS-CoV-2 conform to the central dogma of molecular biology that was coined by Francis Crick?

A: * Central dogma of  moecular biology   * It Explains the flow of genetic information from DNA to RNA…

Q: To clone a bacterial lipase gene into the cloning vector pET28a, you know the full sequence of the…

A: For the ease of manipulation, vectors must be relatively small molecules. They must be capable of…

Q: Why did geneticists believe, even before direct experimental evidence was obtained, that the genetic…

A: Genetics is the study of how traits are passed from one generation to another. This includes but is…

Q: Describe the process of cloning a DNA fragment into the EcoR1and AluI sites of the vector pUC18. How…

A: DNA cloning is characterized as a cycle of creating different duplicates of a specific DNA. This…

Q: what are BIOLOGICAL IMPLICATIONS OF CRISPR-CAS9?

A: CRISPR- CAS9 is used to create animal models to mimic humans especially in case of diseases where…

Q: One problem that researchers sometimes encounterwhen editing genomes with CRISPR/Cas9 is that oneor…

A: The gene therapy or gene editing is an asset for humans, as many of the gene-related disorders can…

Q: In chapter 19 of Watson et al., Box 19-2 explains the ChIP-Chip and ChIP-Seq techniques.  Explain…

A: Transcription Factors (TF) are proteins that binds to specific sequences near a structural gene and…

Q: Design a site directed mutagenesis experiment to convert the wild type GFP sequence into the S65T…

A: Site-directed mutagenesis is the method of DNA (deoxyribonucleic acid) alteration in which a…

Q: What has been the most significant finding of the ENCODE project?Why was the project expanded to…

A: ENCODE stands for Encyclopedia of DNA elements. It was a research project that came after the Human…

Q: What would a scientist need to use CRISPR-Cas system to introduce a specific point mutation into a…

A: When the target DNA is located, Cas9, one of the CRISPR system's enzymes, latches to it and chops…

Q: What is the role of the following molecules in the CRISPR mechanism? *

A: CRISPR stands for clustered regularly interspaced short palindromic repeats. These are a set of DNA…

Q: As we described in class, in the early 1960's Francis Crick and colleagues set out to determine how…

A: Introduction  Genetic code   The sequence of bases that encodes a functional protein molecule is…

Q: Which component of the CRISPR-Cas system directly recognizes thebacteriophage DNA?

A: CRISPR(Clustered regularly interspaced short palindromic repeats) is a region of DNA present in…

Q: the forward primer used in this experiment incorporates part of the cell recognition site, GGCC. How…

A: The TAS2R38 gene in individuals encodes a protein known as taste receptor 2 member 38. TAS2R38 is a…

Q: Basic features of the central dogma of molecular genetics is

A: DNA is a molecule made up of two polynucleotide chains that form a double helix and carry genetic…

Q: Where did the Crispr system first originate and what is its purpose in nature? What is the advantage…

A: We are answering first 4 questions. For rest of questions pls repost.

Q: Based on recent data arising from various animal models, which of the following CRISPR-Cas9 systems…

A: based on recent data arising from various animal models, A. CRISPR-dCas9 systems would be the most…

Q: CRISPR/Cas9 mediated genome editing has provided an unparalleled means of manipulating the genome.…

A: CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats/CRISPR-associated genes) is a…

Q: How must recessive and dominant mutations be treated differently, in theory, when correcting disease…

A: CRISPR Technology uses a combination of 2 types of molecules to edit disease-related genes  A…

Q: elect molecular outcomes that can be achieved with the CRISPR-Cas9 system  (Check all that apply.)…

A: CRISPR- Cas9 is a genome-editing tool which is derived from bacteria.

Q: Genome-wide RNAi screens target expression of > 16,000 genes. Explain how each of these 16,000+…

A: The control of gene expression in a cell to inhibit the expression of a specific gene is known as…

Q: What are some of the scientific and ethical implications of being able to edit the genome with the…

A: Genome editing is a group of technologies to modify genes of organisms and make specific changes in…

Q: The CRISPR-Cas9 system may be able to be used for somatic gene therapy to treat terminal genetic…

A: Mutation: Normal DNA contains a particular sequence of DNA. If the sequence of DNA is changed due to…

Q: Sequencing a genome and identifying individual genes are processes typically carried out  - A. via…

A: The DNA sequencing method is primarily developed by Gilbert and Sanger. Maxam–Gilbert's sequencing…

Q: What are site-recombinases? Describe in detail how cre- recombinase can be used to decipher the…

A: Genetic recombination is the transfer of genetic material between organisms, resulting in offspring…

Q: What technique can you use inorder to get a functional insulin coding sequence cloned into bacteria…

A: Insulin is a hormone produced by the beta cells of islets of Langerhans of pancreas. Insulin is…

Q: A region of the genome is transcribed into a long non-coding RNA. When used as a query, would…

A: BLASTIN matches with the nucleotide query sequence with nucleotide subject sequence. BLASTX matches…

Q: Why was the type II CRISPR-Cas9 system of S. pyogenes selected by several scientists as a model to…

A: CRISPR stands for Clustered Regularly-Interspaced Short Palindromic Repeats, is a revolutionary…

Genomic studies

Genomic studies (genomics) is the study of the whole genome of an organism. Using bioinformatics and high-performance computing, genomic researchers examined massive amounts of deoxyribonucleic acid (DNA)-sequence data to discover variations or mutations that affect drug response, health, or disease. Genomics is a much younger discipline than genetics, having only emerged in the last few decades as a result of technological advancements in DNA sequencing and computational biology.