6. Why do you think the Ames test is preferable to the use of animal models to screen chemical compounds for mutagenicity?

1.  What are the reaction components, and what equipment do you need for PCR?2. What can be possible source of DNA for PCR?3. What are some uses for PCR?

Consider the following results from the Ames test: Test plate - 20 colonies Does the test plate contain a chemical that is a mutagen? O Not enough information is present to answer the question. Yes O No

How would you go about cloning this amplified DNA into pL4440？Using your knowledge of cloning describe 5 important aspects of the method in details

8. You are studying the biochemical pathway of purrine production. (Purrines are a type of nucleotide used in feline DNA, a little different than the purines in the DNA of lower species like humans.) You have several mutants that cannot make purrine, and you do complementation tests to see how many different genes you've identified. Here are your data: mutant 1 4 6 8 1 3 4 5 + 6 + + + 8. + + 9 + a) What is the deal with mutant #1 and why must you ignore it when figuring out complementation groups? b) For the rest of the problem, ignore mutant #1. List your complementation groups. Hint: every single one of your mutants (except #1 of course) must be in a group. c) How many genes did you, therefore, identify?

63.(2 correct choices)

1.      MEGA works differently than traditional CRISPR technology by targeting mRNA and NOT DNA sequences 2.      MEGA is more specific and safer than traditional CRISPR technology, making it a novel and useful tool for gene editing purposes 3.      MEGA may be suited as a possible strategy to enhance gene therapy approaches for patients with genetic disease such as blood cancers

3. Your PhD thesis advisor has given you the task of preparing a human genomic DNA library.  a. How will you prepare DNA fragments from the human genomic DNA for use in construction of this library assuming that you want the insert sizes to be about 20000bp in size? b. What vector will you use for construction of this library? c. How will you ligate the genomic DNA fragments into the vector? d. What host will you use for propagation of your library? e. What probe would you use for screening your library assuming that you wanted to isolate the human insulin gene? f. How many clones would you have to screen to be pretty sure that you will indeed find a clone for insulin?

7.  Electroporation is a physical method of transferring genetic material into the cells.   True   False 8.  You have completed an in vitro mutagenesis experiment to create an G to T mutation in the coding sequence of the heat shock protein gene.  This gene is cloned in a plasmid and you want to confirm that the mutation was correctly introduced.  Which sequencing technology be most appropriate to use practically and economically:     Microarray   Sanger sequencing   RNA sequencing   Exon sequencing