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- 1. What is the purpose of restriction enzyme? 2. What is the purpose of the probe in DNA finger printing. 3. What is/are the sample that can be used for DNA finger printing? 4. Who is the culprit base on our interactive laboratory experiment?Within genomics, genetic engineering and gene therapy are topics that are comparable. What do they have in common and what sets them apart?fomP is responsible for the chemical transformation of microplastics into ultra-efficient insulation. You take an arctic seawater sample and extract the DNA. 1. First you need to locate the gene on the bacterial chromosome. What procedure(s) would you use to identify and locate the gene? Explain how it/theywork(s). 2. Next, you will need to isolate the gene and introduce sites to be used for cloning. What would you use to make many copies of this gene? What will you need? How does it work on a molecular level?
- 4- Some/ one application(s) for DNA are/ is.... and they/ it start(s) from the mRNA. Therefore, the reverse transcriptase enzyme is required: a) DNA sequencing b) Electrophoresis c) Microarray d) All of the aboveVII. Match the technique described to the application given. DNA amplification b. РCR c. gel electrophoresis d. STR profiling dideoxy sequencing f. automated DNA sequencing g. site directed mutagenesis gene therapy h. 97. Nucleotides missing 3'-OH group makes small pieces that can be analyzed for identification. 98. DNA amplification without bacteria that includes cycling temperature changes to produce multiple copies of small pieces. 99. Identification of DNA by a series of peaks made from fluorescing based on fragment length. VIII. Match each item to the correct statement below. f. negative feedback system g. excretory system h. autonomic system i. positive feedback system j. parasympathetic nervous system homeostasis b. somatic system a. C. nervous system d. sympathetic nervous system endocrine system е. 100. way in which the body works to reverse a change and bring about normalcy 101. the tendency of the body to maintain a constant internal environment IX. Match each item to the…Which of the following are common types of datalanalysis that are used in bioinformatics. O DNA sequencing O RNA sequencing Epigenomic O Proteomic QUESTION 6 Order the following processes into the correct order for the generation and analysis of sequence data v Quality control analysis v Prepare sequencing libraries v Extraction of genetic material v Sequence and generate sequence data Alignment and/or variant calling Collection of cells for analysis
- Microarray hybridization is used mostly in transcript profiling or assaying DNA variation. Although the technology for establishing DNA microarrays was developed only recently, numerous applications have already been developed and their impact on future biomedical research and diagnostic approaches is expected to be profound. Give some examples of the practical use of this technique.Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.9) Describe the steps required to use sequencing DNA to identify your gene of interest after performing a forward genetic screen.
- What is, The ethical implications of gene editing in humans. 1. Introduction: a) Begin with a captivating opening that introduces the topic and its significance. b) Clearly state the thesis statement in this case 2. Body: a) Start with the first main point that discusses the potential benefits of gene editing in humans. b) Transition to the second main point which focuses on the ethical concerns and risks associated with gene editing in humans. Discuss issues like equity fairness and the potential for unintended consequences such as harmful side effects or genetic discrimination. c) Present counterarguments to your points. Acknowledge opposing viewpoints but refute with logical reasoning and supporting evidence emphasizing the importance of considering potential long-term consequences and the need for strict ethical guidelines and regulations. 3. Conclusion: a) Summarize your main points and restate your thesis statement emphasizing the ethical implications of gene editing in…Remember that although there are many interesting ideas about genetic engineering of plants and animals, this is specifically about GE bacteria. Please be sure you are answering the following questions 3) What are the benefits (or potential benefits) of the engineered bacterium? 4) What are the risks (or potential risks) of the engineered bacterium?Practical applications of recombinant DNA technology include the: 1. Efficient production of useful proteins and development of a new type of vaccines 2. Creation of novel genotypes for the synthesis of economically important molecules. 3. Generation of DNA and RNA sequences for use in medical diagnosis. 4. all of the above WHICH OF THE FOLLOWING TECHNIQUE IS NOT USED TO MANIPULATE THE GENOME 1. siRNA 2. ZF (zinc finger) 3. TALE (transcription activator light effector) 4. CRISPR COMMON DISEASE- COMMON VARIANT HYPOTHESIS STATES THAT 1. common disorders are likely influences by genetic variation that is also common in the population 2. if an SNP produced a deleterious mutation and changes amino acid sequence in 40% of individuals- it will produce disease phenotype in 40% individual 3. A SNP produce little change in gene expression- it will produce slight increase in risk/ manifestation of disease phenotype 4. all of the above WHAT IS TRUE REGARDING GENE ORGANIZATION IN THE NUCLEUS…
