4.1. Recombinant DNA technology has had a dramatic impact on all aspects of molecular biology, allowing scientist to routinely study cells and their macromolecules in ways that were unimaginable. Describe manipulations central to this technology. (10) 4.2. Draw a diagram to illustrate a technique for creating clones of a particular DNA (DNA cloning).

4.1. Recombinant DNA technology has had a dramatic impact on all aspects of molecular biology, allowing scientist to routinely study cells and their macromolecules in ways that were unimaginable. Describe manipulations central to this technology. (10) 4.2. Draw a diagram to illustrate a technique for creating clones of a particular DNA (DNA cloning).

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter15: Genomes And Genomics

Section: Chapter Questions

Problem 6QP: Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel,...

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1. Given the following advancements in molecular biology and genetics, identify how these contributed/used in recombinant DNA technology: DISCOVERY USE IN RECOMBINANT DNA TECH The "code" like nature of DNA existing as a string of A's, T's, C's, and G's. Discovery of special cutting enzymes that recognize and cut at specific sequences Special DNA molecules that contain an ori site, selection markers, and multiple cloning sites Bacteria can be transformed and can take up DNA from the environment Antibiotic markers and fluorescent genes such as gfp wOT FOR SALE
9) The diagram to the right represents one technique used in biotechnology. The organic compound used to cut the bacterial DNA so that the human DNA could be inserted a(n)
1. TRUE OR FALSE: a) Okazaki fragments are short DNA pieces that explain how the DNA polymerase can continue the synthesis of the new strand. b) Okazaki fragments are short DNA pieces that explain how the DNA polymerase can continue the synthesis of the new strand.
26. You are a forensic scientist investigating a murder for the FBI. Foreign blood was found on the victim's body and you decide to create a DNA fingerprinting profile of the bloodstain along with several suspects. What technology would be best to separate the DNA fragments by their sizes? - [ ] CRISPR - [ ] PCR - [ ] Restriction cloning - [ ] Gel electrophoresis - [ ] Gene therapy 27. An example of gene therapy is: - [ ] Insertion of the insulin gene into the cells of a diabetic person - [ ] Expression of the insulin gene in E. coli. - [ ] Isolation of insulin from a pig. - [ ] Injection of insulin into a diabetic person .
16. Which of the following enzyme repairs the DNA backbone during molecular cloning (that is you are inserting the DNA fragment into a plasmid and the bases in the sticky ends are aligned but the backbone needs to be repaired). a) reverse transcriptase b) restriction enzymes c) DNA ligase d) polymerase
1. For each of the processes below, fill in blanks with one or more of the items in the following list: Ligase (L), Primase (P), enzyme with 5' to 3' activity (E), RNA-dependent polymerase (RNA-DP = polymerase which uses RNA template), DNA-dependent polymerase (DNA-DP=polymerase which uses DNA template). for PCR you need............. for normal mRNA synthesis you need........ for normal DNA synthesis you need........ for normal tRNA synthesis you need....... 2. (please explain) Suppose the DNA synthesis that occurs in PCR is less accurate than cellular DNA synthesis (as in, PCR has a higher error rate than normal DNA synthesis). This difference in error would most likely be because the polymerase used in PCR: a) has a low level of 5' to 3' exonuclease activity b)has a low level of 3' to 5' exonuclease activity c) has a low temp at which it denatures d) adds to the 5' end of the growing DNA chain e) does not use template
1. What is DNA cloning of animals and plants? 2. What is the importance of DNA cloning? 3. Give example or scenario that involves DNA clining (could be taken on the internet). please answer 1,2,3. thank you
1 2 Today's technology has made it easier to quickly and accurately generate DNA profiles. In this part of the activity, you will model the process yourself to solve a crime. Good luck, detective! Crime Report: A thief has stolen a priceless collection of jewels from the Museum of Precious Jewels. Forensic technicians obtained skin cells from a forehead print left on the glass enclosure of the jewel exhibit. DNA has been isolated and PCR amplified for some of the standard STR loci. A partial genetic profile generated from the collected DNA is shown in Figure 5. 10 50 DNA Profile from Forehead Print Number of base pairs 00 50 40 D58818 075830 I 16 MU DES1179 Shandand (10) 70 CSF1PO DITS820 80 100 Figure 5. The DNA profile of the forehead print from the scene of the crime. Each colored line shows the alleles for one of four of the core CODIS STR loci (D5S818, CSF1PO, D7S820, D8S1179). and data for the four STR loci that were included in the A suspect was identified in the case. Her DNA…
1. To study or edit a single gene, scientists must first isolate it from the rest of the genes in a genome.2. They may start by cutting an organism's DNA and then cloning all of the fragments at the same time.3. As a result, a genomic library is created, which is a collection of clones that collectively contain all of the DNA in a genome. Which of the following statements is/are true ? a. 2 and 3 b. 1,2, and 3 c. 1 and 2 d. 2 e. 1 f. 1 and 3
1. How do we describe the two strands of DNA, once they have separated during denaturation? 2. Polymerase Chain Reaction requires both forward and reverse primers. Where do each of these primers bind? 3. What is the purpose of the primers in a PCR reaction? 4. PCR separates molecules using what property of DNA? Based on this, which direction do DNA molecules travel through a gel? (to the positive end? Or the negative end?) 5. What does ‘qPCR’ stand for and how is it different from regular PCR?
4. Explain the importance of primers in pinpointing the region of DNA to be amplified, and discuss why primers make PCR such a powerful and useful process. 5. In PCR, what replaces the need for helicase? In PCR, what replaces the need for primase? In PCR, why doesn't the DNA polymerase denature under the high temperatures of denaturation?
1. DNA plays an important role in two processes. During the process of replication, dna provides information to copy itself, so genetic information can be passed on from one generation of cells to the next. DNA also provides instructions for making proteins, in these instructions are vital to the maintenance and function of cells. DNA provides the information on how to order the amino acids required for making various proteins. Describe the significance of crude extraction of DNA from different sample organismic. 2. Distinguish the importance of the following theorems that are interrelated to the study of bioinformatics: (1) proteomics, (2) genomics, (3) biotechnology, (4) biostatistics, and (5) biological engineering. 3. Describe the procedures that you have encountered in the bacterial identification virtual laboratory (media.hhmi.org). Describe each part of the procedure: (1) simple preparation, (2) PCR amplification, (3) PCR purification, (4) sequencing preparation, (5) DNA…