4. With the use of P22 as a generalized transducing phage grown on a pur+ pro* his+ bacterialdonor, a recipient strain of genotype pur pro his was infected and incubated. Afterward,transductants for pur+, pro*, and his* were selected individually in experiments I, II, and III,respectively.a. What medium is used in each of these selection experiments?b. The transductants were examined for the presence of unselected donor markers, with thefollowing results:IIIIIIprohis86%pur his44%pur pro20%pro+ his0%purt his0%pur pro14%pro his+10%pur his54%pur prot61%pro+ hist4%purt hist2%pur prot5%

a. The material that was utilized in the selection experiments: I. The selection of purified…

Answered: 4. With the use of P22 as a generalized…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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2. You are planning to clone a gene into the PBR322 plasmid vector. The gene has Bgl II restriction enzyme sites on both the 5' and 3' ends. Restriction enzyme Recognition site 5'... CTGCAG...3' 3... GACGTC... 5 5... GAATTC...3' 3:... CTTAAG. ECORI Hindil Pst I BamH Eco RI 5' Tetracycline Resistance Hind III 5...AAGCTT...3' PBR322 (4.36 kb) Xmalll 3...TTCGAA ... 5' 5... GGATCC...3' 3... CCTAGG...5' 5...CCCGGG...3° 3...GGGCCC ... 5 5... A'G ATCT...3 3... TCTAGA... 51 Bam HI Origin Replication Xma l BglI E. coli PBR322 plasmid showing restriction sites and resistance genes. Pvull With which restriction enzyme would you cut the PBR322 plasmid vector? Give a reason for your answer. You assemble a ligation reaction to join the gene to the plasmid DNA. What types of product would you expect to obtain from the ligation reaction? Please consider the selection marker genes, Ampicillin resistance gene and Tetracycline resistance gene. What would the antibiotic resistance phenotype be of a…
1. A set of 4 Hfr bacterial strains were used in a time-of-entry experiment to map the locations of nine genes (A, B, C, D, E, F, G, H, I). The experiment was terminated after 22 minutes, which did not provide enough time to map all 9 genes with a single Hfr strain. Using the data on the amount of time it took to transfer each gene to a recipient during conjugation (see table below), draw a circular map of the genes on the bacterial chromosome. Your map should also indicate the Hfr origins of transfer with arrows. You don't need to indicate relative distances between genes, but you should use the given times of entry to figure out relative gene order. G H n/a 13 n/a 9 в с n/a n/a n/a |13 n/a 17 n/a 14 n/a n/a| 12 А D E F I Hfr 1 Hfr 2 1 22 10 n/a n/a n/a 2 n/a n/a |8 n/a n/a n/a 11 Hfr 3 n/a 4 Hfr 4 n/a n/a 2 18
0.9kb dna fragment inserted into GEM1lr.colonies modt likely...... light blue blue white no colonies ......... used to confirm presence of 0.9 kb . dna sequencing Pcr microarray a and b
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One of the following statements is FALSE A. Labeled antibodies are the tagged probes of Southern blotting and ELISA (enzyme linked immunosorbent assay) B. In biopharmaceutical engineering; immunogenicity and pharmacokinetic properties are altered favorably; hence therapeutically C. In RFLP (restriction fragment length polymorphism), number and length of restrictionfragments vary due to genome polymorphism and bases sequencevariations D. In gene replacement therapy a normal gene allele substitutes permanently a mutant allele in a somatic cell
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