4. With the use of P22 as a generalized transducing phage grown on a pur+ pro* his+ bacterialdonor, a recipient strain of genotype pur pro his was infected and incubated. Afterward,transductants for pur+, pro*, and his* were selected individually in experiments I, II, and III,respectively.a. What medium is used in each of these selection experiments?b. The transductants were examined for the presence of unselected donor markers, with thefollowing results:IIIIIIprohis86%pur his44%pur pro20%pro+ his0%purt his0%pur pro14%pro his+10%pur his54%pur prot61%pro+ hist4%purt hist2%pur prot5%

a. The material that was utilized in the selection experiments: I. The selection of purified…

Answered: 4. With the use of P22 as a generalized…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Similar questions

If a researcher accidentally transformed the bacterial two hybrid plasmids ( pKT25-X and pUT18-Y) into a normal strain of E coli that produces adenylate cyclase, his/her experiment will: a. show a high lacZ activity in all tubes (control and experiment) because Proteins X and Y do not need to be interacting in order to activate lacZ gene prescription. b. not work because cells lack cAMP. c. need replicates in order to show true interaction between X and Y.
Match description with Term: 1) determine the amount of protein in a sample 2) determine the location of a protein in a cell or sample 3) determine the amount of transcript in a sample 4) Determine the location of a transcript in a sample or cell a. In situ hybridization b. northern blotting c. western blotting d. immunocytochemistry
7) The figures below depict nine interacting microbes. The center cell (black circle) is the altruist. Individuals that receive benefits from the altruist have white crosses. Dark blue individuals are clonemates. Individuals with small red circles are cooperators. Small green or yellow circles indicate different molecules on the surface of the cell. Which of is consistent with a greenbeard effect (phenotype matching)? the figures below a) A A d) D e) all of the above DO B В +4 ++ + D +
Phage Mu may be integrated into E. coli genomic DNA via Select one: O a. recombination catalyzed by homologous recombination enzymes. O b. conservative transposition O c. integration catalyzed by integrase. d. replicative transposition
please answer the multiple choice questions no deailed answer needed but answer both parts thanks
Describe the differences between the F factor and Hfr transfer. Include how the donor and recipient are affected in each type of conjugation.
Assume you have successfully cloned a small (200 bp) fragment of DNA into the polylinker region of a pUC18 cloning vector. Describe the appearance of transformed colonies you would expect to see on each of the following plates: plain media, media containing ampicillin, media containing tetracycline, media containing ampicillin and X-Gal.
Describe the fate of the λ phage during the infection process with mutants in the following genes: CI CII CIII N Cro att Q Describe the fate of the λ phage during the infection process with mutants in the following areas: OR1 OR3 PL PR PRE PRM tL1 tR1
In a hypothetical situation, the gene for tetracycline resistance is located on a plasmid within a particular bacterium. This bacterium readily forms a pilus and donates one strand of the plasmid to another bacterium. What do you know about the bacterium with the tetracycline resistance? O It cannot receive a plasmid from another bacterium. O It must have the F factor gene because it can perform conjugation. O If it donates the plasmid, it will lose its own tetracycline resistance. O It has the Hfr gene and it can participate in transformation.
Both viruses and short bacterial fragments from lysed bacteria can pass through the filter in the U-tube. Would this make it impossible to differentiate between transduction and transformation if some prototrophs were produced by the U-tube experiment with E. coli bacteriophages present? Why or why not?

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