4. Which step consists of purification and buffer exchange?

1. Describe how you would go about making up the following solutions. a) 150ml GTE buffer (resuspension solution) containing, 20mM Tris pH 8, 5000µM EDTA, 1M Glucose

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3. What is the importance of buffering in cells and tissues?

10. Why would it be inaccurate to say that a buffer is a solution that maintains a constant pH? K/U

6. Of the following pairs write which ones could be used as buffers and why: (a) 0.1 M NaOH and 0.1 M NaCH3COO, (b) 0.01 M H2SO4 and 0.01 M K2S04, (c) 0.01 M NH3 and 0.02 M NH4CI, (d) 0.2 M HNO2 and 0.2 M NaNO2.

4. Tabulate the similarities and differences between an acidic and basic buffer solutions

II. Was the buffer better at providing H* or binding H* ?. Explain your answer.

1. For each of the following separation methods covered in class, describe the basics of how that technique works, and what difference in material properties is used to separate molecules by that technique (for example: molecular size/MW, solubility differences, surface charge, ...) ultrafiltration, centrifugation, size-exclusion chromatography, isoelectric focusing. reverse osmosis, adsorption (physisorption), ion exchange, dialysis, affinity chromatography, electrophoresis and

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