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- 1. Adding as little as 0.1 ml of concentrated HCl to a liter of H20 shifts the pH from 7.0 to 3.0. Adding the same amount of HCl to a liter of a solution that 0.1 M in acetic acid and 0.1 M in sodium acetate, however, results in a negligible change in pH. Why do these two solutions respond so differently to the addition of HCI?8. Explain how buffer systems are important in organisms. In the human body, bicarbonate and carbonate ions work together to stabilize or buffer the pH of body fluids. What would happen to your blood if these buffering agents were removed? Use the buffers assignment to help you4. A 100-mL buffer solution with pH of 4.80 is prepared as a stock solution. Using this stock buffer solution, three buffers are prepared by diluting a given volume of the stock buffer (as shown below) to 100 mL with water. Volume used from Buffer Final volume, mL original solution, mL 1 5 100 2 10 100 20 100 a. Do they have the same pH? Check the box corresponding your answer. O Yes O No HY 47.1 Basic Biochemistry, Laboratory b. Which is true about their absolute concentrations? Check the box corresponding your answer. [Buffer 1] > [Buffer 2] > [Buffer 3] [Buffer 3] > [Buffer 2] > [Buffer 1] [Buffer 3] = [Buffer 2] = [Buffer 1] [Buffer 3] = [Buffer 2] > [Buffer 1] [Buffer 3] > [Buffer 2] = [Buffer 1] c. Which is true about their concentration ratios? Check the box corresponding your answer. [Buffer 1] > [Buffer 2] > [Buffer 3] [Buffer 3] > [Buffer 2] > [Buffer 1] [Buffer 3] = [Buffer 2] = [Buffer 1] [Buffer 3] = [Buffer 2] > [Buffer 1] [Buffer 3] > [Buffer 2] = [Buffer 1] %3! %3D d.…
- 3. Normally the pH of the human body is fixed in a very narrow range between 7.35 and 7.45. A patient with an acidotic blood pH of 7.3 may be treated with an alkali such as sodium hydrogen carbonate. Why would this treatment raise the pH of the blood?5. A 60-year old woman was admitted to the hospital with a diagnosis of pneumonia. She had beenon a thiazide diuretic for 6 months previously for treatment of heart failure. Her lab results were:K+ 2.1 mMpH 7.64PCO2 32 mm Hg PO2 75 mm Hg HCO3- 33 mMClassify her acid-base disturbance. Hint: graph it out on the pH-HCO3- diagram2. You are given 575mL of a .200M solution of NaOH and are adding 600mL of water What is the new concentration?
- Which pH value indicates a solution that has more hydrogen ions that hydroxide ions? pH 9 pH 4 pH 7Page 16: Draw a picture of a pH scale. Be sure to label the following terms or your picture: acid, base, neutral, high concentration of hydrogen (H*), & low concentration of hydrogen (H+).4. Tabulate the similarities and differences between an acidic and basic buffer solutions