-35 sequence -10 sequence +1 Transcribed lac operon TTTACA N7 TATGTT NG A lacl GCGCAA N7 CATGAT N, Al trp operon TTGACA N7 TTAACT N, A rrnX TTGTCT N16 TAATAT N7 Al TTGATA N46 TATAAT N, A recA lexA TTCCAA Ny7 TATACT NG A TRNA" TITACA N16 TATGAT N, Al Consensus TTGACA TATAAT sequence

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter29: Transcription And The Regulation Of Gene Expression
Section: Chapter Questions
Problem 17P
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Let’s suppose a DNA mutation changes the consensus sequence at the −35 site in a way that inhibits σ factor binding. Explain how a mutation could inhibit the binding of σ factor to the DNA. Describe two specific base substitutions you think would inhibit the binding of σ factor. Explain why your base substitutions would have this effect.

-35 sequence
-10 sequence
+1 Transcribed
lac operon
TTTACA N7 TATGTT NG A
lacl
GCGCAA N7 CATGAT N, Al
trp operon
TTGACA N7 TTAACT N, A
rrnX
TTGTCT N16 TAATAT
N7 Al
TTGATA N46 TATAAT N, A
recA
lexA
TTCCAA
Ny7 TATACT NG A
TRNA"
TITACA N16 TATGAT N, Al
Consensus
TTGACA
TATAAT
sequence
Transcribed Image Text:-35 sequence -10 sequence +1 Transcribed lac operon TTTACA N7 TATGTT NG A lacl GCGCAA N7 CATGAT N, Al trp operon TTGACA N7 TTAACT N, A rrnX TTGTCT N16 TAATAT N7 Al TTGATA N46 TATAAT N, A recA lexA TTCCAA Ny7 TATACT NG A TRNA" TITACA N16 TATGAT N, Al Consensus TTGACA TATAAT sequence
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