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- Test Tube # Enzyme 1 (uL added) Product Formation (umol) 1 0 0 2 25 0.08 3 50 0.15 4 75 0.22 5 100 0.3 6 125 0.37 7 150 0.45 8 175 0.53 9 200 0.59 10 225 0.68 11 250 0.74 A. Make a graph is Excel/Google Sheets plotting Product formation (umol) on the y-axis and Enzyme volume added on the x-axis. Paste the graph below. Make sure to have axis labels and a title on the graph. B. What can you conclude from this graph, ie how is the amount of product formed changing as a function of the amount of enzyme present?answer the following questions 1.What is the wavelength used for the spectrophotometer? What is the principle of the spectrophotometer? 2.What is the reagent used to detect glucose in the oh and temperature experiments? What is its principle?3.What is the optimum temperature for invertase reactions?4.What is the optimum ph for the invertase reactions?Below is a simple set of weights obtained while immersing potato slices in various sugar solutions over 30 minutes. Please show all step by step calculation calculate the rate of weight change over this 30-minute period Write your answer in standard notation and use 3 digits past the decimal point - e.g. 0.005, 0.030. (Note that the last zero in an answer is considered to be a digit). 0.0 M sucrose = _______ 0.008_____________ g/min 0.4 M sucrose = _______ -0.013_____________ g/min 0.6 M sucrose = ________ -0.023g____________ g/min 0.8 M sucrose = ________-0.026____________ g/min 1.0 M sucrose = ________ -0.033____________ g/min Can i please have the step by step calculations Using the osmosis data in the question above, determine the percent weight change for each sucrose solution . Write your answer in standard notation and use 1 digit past the decimal point - e.g. 8.6. (Note that the last zero in the answer is considered to be a digit) 0.0 M sucrose = _________…
- Below is a simple set of weights obtained while immersing potato slices in various sugar solutions over 30 minutes. For each solution, calculate the rate of weight change over this 30-minute period. standard notation and use 3 digits past the decimal point 0.0 M sucrose = ____________________ g/min 0.4 M sucrose = _____________________ g/min 0.6 M sucrose = _____________________ g/min 0.8 M sucrose = _____________________ g/min 1.0 M sucrose = _____________________ g/min Using the osmosis data in the question above, determine the percent weight change for each sucrose solution standard notation and use 1 digit past the decimal point - Show your work in the space below. 0.0 M sucrose = ____________________% 0.4 M sucrose = _____________________% 0.6 M sucrose = _____________________% 0.8 M sucrose = _____________________% 1.0 M sucrose = _____________________% Time (min) 0M sucrose 0.4M sucrose 0.6M sucrose 0.8 M sucrose 1.0M sucrose 0 4.36 g…45. What happens to CO2 levels with increasing sugar concentration? Is this pattern observed for all sucrose treatments? 46. Based on your results, can you explain which ingredients are essential for fermentation to occur and why? 47. Did your observed results reflect your predictions for the circumference of the balloon? 48. Do you reject or fail to reject your null hypothesis for the circumference of the balloon? Explain. 49. Did your observed results reflect your predictions for the percentage of alcohol? 50. Do you reject or fail to reject your null hypothesis for the percentage of alcohol? Explain. 51. Identify any possible sources of error that may have affected the results of this experiment. 52. Using Excel, make a bar graph with the results of the circumference of the balloon and the percentage of alcohol side by side.Safari File Edit View History Bookmarks Window Help ... く 令 Thu Feb A savvasrealize.com D Home - Student Portal ASavvas EasyBridge く Exit Interactivity: Comparing Cellular Respiration and Fermentation Savvas Realize Due 02/13/22 11:59pm Comparing Cellular Respiration and Fermentation 3. Based on the images in the slider, how does cellular respiration compare with lactic LACTIC ACI FERMENTATION acid fermentation? Glucose CYTOPLASM 000000 NAD ADP NAD* cycles NADH ATE back ATP Pyruvic A cid A. Both use enzymes from the 000 mitochondria. NADH B. Both produce equal amounts of NAD+ 000 000 Lactic Acid ATP. Lactic Acid Fermentation C. Both use NAD+ electron carrier molecules. D. Both release carbon dioxide. Check Answer < 6 of 7 10 étv MacBook Pro く く※ 23 2$ 3 4 5 7 CO
- Lab: Isolation of beta-amylase from sweet potato Objective: To isolate the enzyme β -amylase using sweet potato as a source Materials Required: Sweet potato.Knife/peeler.Mortar and Pestle.A Blender.Blue capped tubes.20mM sodium Phosphate buffer at pH 7.Vortexer. Procedure: 1.Take a clean sweet potato and peel the skin off.2.Weigh the peeled sweet potato and note the weight.3.The sweet potato is cut into small pieces and transferred into a mortar and pestle.4.The pieces are crushed and then transferred into a blender.5.Add 40 ml of cold 20mM sodium phosphate buffer saline. Blend it until it forms a paste.6.Gently transfer the potato slurry into a blue capped tube.7.Allow the enzyme to extract over a 1 hour period at room temperature, with frequent vigorous stirring on a vortex mixer.8.Then the extract is filtered using a GF A glass fibre filter and the filtrate is collected in a new blue capped tube.9.Centrifuge the filtrate at 12000rpm for 20 minutes at 4 degree Celsius.10. After…Please answer these question. I need these for lab. PRE-LAB ASSIGNMENT – (YOU WILL NOT BE ALLOWED TO BEGIN WORK ON LABWITHOUT THE COMPLETED CALCULATIONS) 1. COMPLETE ALL CALCULATIONS REQUIRED FOR TABLE 1 ( For all measurements, the [PNPP] concentration will be 600 μM in a 1 ml final volume.) PNPP (substrate) stock solution: 100 mM PNPP in buffer 2. IN YOUR LAB NOTEBOOK, DRAW YOUR PREDICTION OF WHAT THEFOLLOWING GRAPHS MIGHT LOOK LIKEa. pH vs. V0b. TEMP vs. V03. OUTLINE THE BEST/MOST EFFICIENT WAY TO PREPAREConvert the experimental glucose concentration from mM (millimolar) to g/100 mL. 1/100 Dilution factor, Assume 1L of Solution. Show ALL Steps 2.340 mM
- Below is a simple set of weights obtained while immersing potato slices in various sugar solutions over 30 minutes. For each solution, calculate the rate of weight change over this 30-minute period. wite your answer in standard notation and use 3 digits past the decimal point - e.g. 0.005 Please show the calulations on how you got the answer 0.0 M sucrose = _______ ____________ g/min 0.4 M sucrose = ________ _____________ g/min 0.6 M sucrose = _____________________ g/min 0.8 M sucrose = _________ ___________ g/min 1.0 M sucrose = __________________ g/min Using the osmosis data in the question above, determine the percent weight change for each sucrose solution use 1 digit past the decimal point - e.g. 8.6. 0.0 M sucrose = ________________% 0.4 M sucrose = ___________________% 0.6 M sucrose = __________________% 0.8 M sucrose = __________________% 1.0 M sucrose = __________________% percent change data, determine the concentration of sucrose that would be isotonic.…Question: Determine the amount of dehydrated medium needed to prepare 15 butts, 20 slants, and 25 butt-slants. Include amount for 2 additional units of each as excess to compensate for compounding losses.CELLULAR RESPIRATION AND FERMENTATION Name: Date: Instructor: Section/Group: POST-LAB QUESTIONS 1. Both cellular respiration and fermentation ordinarily begin with which molecule? ves 2. What role does cellular respiration play in the metabolism of an organism? 3. Glucose breakdown results in the breaking of C-H bonds and stored energy is released. Compare and contrast the end products of fermentation and cellular respiration in terms of their energy produced. 4. Fermentation results in the net production of only 2 ATP, while cellular respiration results in the production of at least 36 ATP. Explain these results with reference to the end products of both of these processes. Cellular Respiration and Fermentation 93