3. Briefly describe the cytogenetic method that was used in the following photo. A 3 X Y

4. Using the information in problem 3, make another plot showing how DX and DP (rates of production of cells and product) vary with D (on the same graph), covering the same range of dilution rates in problem 3. 5. Genetics & Gene Control A. Explain the difference between a DNA strand, a chromosome and a gene. B. Would a cell with a deletion or a point mutation be more likely to revert back to the original phenotype? Why? C. Define feedback repression. On a basic level, how does this work in a cell?

need correct answer

e. You also study the expression of a different mutants for this gene. For each mutant answer the following: Does this mutation change the sequence of the protein produced? Why or why not? If it does change the sequence of protein be sure to write out the new sequence. If it does not change the protein sequence, what effect (if any) would you expect it to have on expression of the gene? 1 20 ORI 40 60 5' ..TTCGAGCTCTCGTCGTCGAGATACGCGATGATATTACTGGTAATATGGGGATGCACTATC...3' 3'...AAGCTCGAGAGCAGCAGCTCTATGCGCTACTATAATGACCATTATACCCCTACGTGATAG...5’ promoter

Mention different roles & applications of polymerase chain reaction. Please  briefly explain at your own words.

3. Briefly describe the process of creating an induced pluripotent stem cell. a. What was the dogma or principle truth that was proven wrong by this process?

1. Describe the basics of flow cytometry. How is this technology used to sort cells? 2. Describe an experiment demonstrating the utility of cell sorting in biotechnology. 3. How do you think flow cytometry can be used as a medical application? What are some examples where flow cytometry is used in the treatment or diagnosis of specific diseases?