23. Describe each type of transposon and how they integrate into the DNA. How are transposons responsible of antibiotic resistant bacteria?

39.  Which of the following are general features found in a plasmid used for bacterial cloning? Group of answer choices Origin of replication Multiple cloning site Multiple cloning site and antibiotic resistance gene Multiple cloning site, antibiotic resistance gene, and origin of replication Locus of transformation Locus of transformation, multiple cloning site, and antibiotic resistance gene Antibiotic resistance gene

1. Determine the effect of the following mutations on the DNA sequence. In each case, the mutation is described after the sequence (REFER TO THE SUPPLEMENTAL DOCUMENT FOR GUIDANCE TO THIS QUESTION). Adenine nucleotide (A shown in red below) was inserted into the DNA sequence at the position indicated by the arrow). Write out the sequence of the mutated DNA and the protein made from it. What is the effect of this mutation on the protein? (For example, how will the mutation affect the length and sequence of the protein? What about the function of the protein?) A 3' TACATGG'TTGTGCTAATT 5'

. All mutations that abolish function of the Rho termination protein in E. coli are conditional mutations;no cells with null mutations of the Rho-encoding genehave ever been isolated. What does this tell you aboutthe rho gene and its product?