2:16 Bacterial Growth Lab Back General Microbiology_23210 Use this data: time 30 oC 37 oC Minutes) OD 550 nm OD 550 nm 0.05 0.05 20 0.06 0.07 40 0.1 0.21 60 0.16 0.44 80 0.28 0.95 Graph your data on semi-log paper so that you get a straight line during the log phase. semi log_numbered[1]_(2).pdf As an alternative, I highly recommend using a spreadsheet- Excel, Google sheets-to plot the data. I will work on a tutorial for this. 4- Record your data in your lab notebook. Keep the graphs handy. Dashboard Calendar To Do Notifications Inbox
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![2:16
Bacterial Growth Lab
Back
General Microbiology_23210
Use this data:
time
30 oC
37 oC
Minutes)
OD 550 nm OD 550 nm
0.05
0.05
20
0.06
0.07
40
0.1
0.21
60
0.16
0.44
80
0.28
0.95
Graph your data on semi-log paper so
that you get a straight line during the
log phase.
semi log_numbered[1]_(2).pdf
As an alternative, I highly recommend
using a spreadsheet- Excel, Google
sheets-to plot the data. I will work on a
tutorial for this.
4- Record your data in your lab
notebook. Keep the graphs handy.
Dashboard
Calendar
To Do
Notifications
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- Escherichia coli Mycobacterium phlei Bacillus Micrococcus subtilis luteus A B Figure 1: Agar Slant Cultures of Bacteria (Gary E. Kaiser, Ph.D.- Professor of Microbiology) . Observe and describe the colour of the slant cultures (A-D) in fig 1. ( . Define the following terms: pure culture, sterile medium, inoculum, aseptic technique, and colony. What is the name of the cultures that you used . Where they gram negative or positive Define the following terms: psychrophile, mesophile, thermophile, and hyperthermophile.I U Cardo 12 1三 ニ 三 rI TI3 4. 111 I. 5. What are the basic shapes of bacterial cells? What are the basic Know the terms. Which types of organisms have axial filaments? How would classify the following in terms of gram stain reaction and shape and arrangement of the cells Escherichia coli? Staphylococcus aureus? Streptococcus pyogenes? Bacillus anthracis? Do these organisms cause any important diseases? arrangements? you IIIKemaining Time: 1 nour, vo * Question Completion Status: 10 20 3r 4 21 22 23 24 5 6 7 8 9 Multiple Answer: Choose all correct answers 10 11 the lag phase is associated with the highest levels of bacterial growth ✓the bacterial growth curve has several phases including the lag, log and death phases Othere is no binary fission occuring during the stationary phase O elongation is one of the first steps in bacterial reproduction Ogrowth curves are often represented logarithmically diluting colonies can help you to quantify how many bacteria were present in your original culture the Log phase is associated with rapid rates of DNA replication Filtration is a technique that can help to measure microbial death 12 13 14 A Moving to another question will save this response 49°F Mostly sunny Q Search 15 16 17
- 6.0 EXPECTED RESULT FOR BACTERIAL GROWTH CURVE Table 1 Culture 1,0ptical Density (OD 550) 0.07 0.07 Sampling Time, Minutes 30 60 0.08 90 0.10 120 0.13 150 0.42 180 0.78 0.92 210 240 1.02 270 1.07 300 1.17 330 1.22 360 1.27 390 420 1.27 1.28 Please plot a graph culture 1, OD 550 versus sampling time, minutes. Then discuss a phases of bacterial growth curve completely.8. A. Use Excel (or another graphing program) to draw the growth curve, In (X/X.) vs time, for bacteria grown in a 20 L suspension cell culture, given the following data: - initial concentration: 0.120 gdw cells/L Also report: - lag time: 1.5 hours - mass doubling time during exponential growth: 250 minutes - duration of exponential growth phase: 1 day (24 hours) - negligible time in the deceleration phase - 13 hours in the endogenous metabolism phase with no change in cell concentration - cell death rate with k = 0.0178 min -¹. B. What is the specific growth rate, µ? C. What is the maximum concentration of cells in the reactor? (gdw cells/L) and when does this occur? D. Other than time zero or the end of lag phase, at what time is the concentration of living cells in the reactor equal to the initial concentration of 0.120 gdw/L?Calibri Light (H.. v 11 A BIU ov Av A 2. Are bacteria unit- or multicellular? What about the chains or colonies seen in slides and. ヘ三 diagrams? Explain. A. Bacteria also play a role in the maintenance of healthy ecosystem. They help decompose decaying matter and help digest food in the guts of animals. 3. If a person is diagnosed with syphilis, which bacteria species is most likely the cause? A. Syphilis is usually a sexually transmitted disease, but it is occasionally acquired by direct nonsexual contact with an infected person, and it can also be acquired by an unborn fetus through infection in the mother 4. Think about Clostridium botulinum. Explain the terms below that describe this species. a. Gram-positive b. Anaerobic C. Motile d. Botulism 5. Explain why methicillin-resistant S. aureus (MRSA) is a problem in clinical medicine. 6. Do you see any nuclei in the specimens? What accounts for the colors of the cells? Explain. 7. Explain gram stain method in terms of how useful it is…
- = Q Set up Bacteria Growth Experiment 2024 Saved Search Design Layout References Mailings Review View Help Table Design Layout (Body) 11 V A A Aa PEET I Uab x, x AA Font √√ Data Collection Table 1-3: just bacteria sample Day General Observations (color, texture, coverage) 2 No obvious growth. 6 Paragraph V עו # of % Photo of plate colonies Coverage 0% Colors range from white, to light grey, size being roughly ½ cm circles. 1-3 3.65% 2-3 3-3 Large amount of growth since day 4 circles ranging from white grey, black, green, and pink have been observed. White and black areas are about 2 1/2cm around while pink, and green are 1/2cm around. There is also many small white dots covering the majority of the surface area. LX Text Predictions: On Accessibility: Investigate 75° COLLEGE 1-4 40% 2-4 3-3 W FB B Normal No Spacing Heading Heading 2 Title Subtitle Subtle Emphas F7 FB SAMSUNG >/11 D Styles 8 Circles ranging from white grey, black, 1-5 green, and pink have been observed getting bigger.…es/_518299_1/cl/outline?customClassicLocation =%2Fwebapps%2FBb-McGraw Hill-BB5744b9beb8ccb%2Fapp%2Flink%2Finbou Edits will not be automatically saved. Save now File Edit Format Tools Help Chapter 7 List Paragra... functions? ● Unsaved ● ● Calibri ● What types of nutrients do microbes require, and where are they obtained? What are the different nutritional types, and where do they obtain their carbon and energy? What are the various types of passive and active transport, and how do they work? How do various environmental factors affect microbes, and how are they classified based on these factors? T 11 Y BIU A - A What are the five ecological associations between microbes, and how do they work? How do microbes grow and divide, and what are the stages of a population growth curve? Chapter 8 What is metabolism, and what are the two general types of metabolic reactions? 0 Mar 12 111 !!! 8:08 A OA serial dilution of a bacterial culture yields the following number of colonies. Which plate(s) should be used to determine the original cell density? Plate A Plate B Plate C Plate D Plate E Dilution Factor 10-5 10-6 10-7 10-8 10-9 # of Colonies Too many to count 850 456 80 14 Group of answer choices All of these choices A & B D & E E None of these choices A B & C D B C & D C
- Im 100 4. You have a culture of yeast that is at a density of 8x10' cells/mL. You dilute the sample 1:10, then 1:1,000 again, and finally you dilute the sample an additional 1:5. You add 0.1 mL of the final dilution to a spread plate. Assuming that most of the cells in the original culture were living, how many colony- forming units do you expect to count on your pour plate the next day?This is how a hanging-drop slide is prepared. Figure from Macedo, Wikimedia Commons, 2016. 2 3 4 slide cover slip vaseline concavity drop of microbiological culture CA inoculation loop 11. Which slide gives you more information, the hanging drop or the stained slide? Why do you say so? 12. Why might it be valuable to know whether a bacterium is motile?TSA plates of microorganisms grown at 30°C or 37°C In words, describe the differences you see for each organism (E. coli M.,luteus ,S. marcescens and S. saprophyticus) grown at 30°C or 37°C (ie, amount of growth, color, etc