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First page is reference for number 4-7
![PART A: INOCULATION ANALYSIS
[Reference the Analysis instructions of the SRB Inoculation assay protocol.]
1. What is the Average, Standard Deviation (SD), Coefficient of Variation (CV) and %CV for each
cell density group for your data?
Cell
Density
AVG
SD
CV
%CV
A
B
C
D
E
100,000 50,000 25,000 12,500 6,250
2. What is the equation of your regression line?
3. What is the r² value for your data?
F
G
H
3,125 1,500 0
DIAM 10 MURSY 1010
UDW JN
IDW
4. Using the values above, determine the optimal cell number to plate for cytotoxicity assay. This
should be done based on the combination of two factors:
1. Low CV value
2. Linearity (r2 value)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F4fe4c5ff-3153-480d-9c91-a45f42309b22%2F5d0929d0-e961-498e-a3ba-28f5baa7c9c0%2F7v5sh8i_processed.jpeg&w=3840&q=75)
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- Please ASAP. Thank you. The following diagram represents the results of hemoglobin samples of 5 patients (1-5) that were run on an electrophoresis gel at pH 9.2. The first 3 lanes show the control samples, for comparison. Consider Patient 4 and Patient 5. If patient 2 is female and patient 5 is a male, and they conceive a child together, what is the chance that the child will have sickle cell anemia (the disease)? Give your answer as a percentage (%)2. The following data were generated after a set of bovine serum albumin standards were measured via the BCA assay. Table 1. Absorbance measurements at 562 nm for BSA standards measured in triplicate in a BCA Assay. Standards A B C D E F G H I Conc (ug/ml) 2000 1500 1000 750 500 250 125 25 0 Replicate 1 Abs @ 562 nm 2.381 2.039 1.505 1.143 0.819 0.454 0.245 0.056 0 Replicate 2 Abs @ 562 nm 2.456 2.052 1.489 1.156 0.823 0.46 0.272 0.049 0 Replicate 3 Abs @ 562 nm 2.43 2.05 1.47 1.17 0.81 0.48 0.255 0.053 0.00 Use Excel or your graphing program of choice to create a standard curve using the data from table 1. Consider the best way to graph this data. Make sure the type of graph that you select will allow you to add a line of best fit. Display the equation for the line of best fit directly on the graph. Hint... you'll want to think about how to treat the triplicate values when making the graph. Also consider the type of graph that should be selected to allow for addition of the line of…Download BLOSUM30 and BLOSUMB0 substitu- tion matrices and place them side by side on your computer screen. What are the differences between the two matrices? Why do you see these differences?
- Bradford Assay Fill in the average A595 and A595 of sample minus A595 of blank. Show some sample computation. Concentration (micrograms/ml) A595 - trial 1 A595 - trial 2 A595 - trial 3 average A595 A595 of sample minus A595 of blank 0 1.501 1.446 1.447 2 1.624 1.558 1.559 5 1.731 1.749 1.712 10 1.901 1.838 1.892 15 2.161 2.108 2.228 18 2.231 2.277 2.3193. In table 2 below, record the number of corn kernels that are smooth and rough. Table 2: Number of kernels Texture smooth rough Total 4. Compare your observed and expected results by doing the chi-square test (Table 3). Notes on calculating the chi-square number Obtain your observed values (counted number of smooth kernels, counted number of rough kernels) from Table 2 above. Number of Kernels 215 Calculate your expected (e) values based on your predicted phenotypic ratio and using the total number of kernels calculated in Table 2. For example, if your predicted ratio is 1:1 (smooth: rough) and the total number of kernels obtained is 600, your e value for smooth kernels would be 300 (1/2 x 600) and your e value for rough kernels would also be 300. 01.22 (td) The symbol > means the sum of. Therefore, you need to add all the d²/e values to get your chi- square (X2) number. 35 150 ceetb Table 3: Chi-square calculations Observed value (o) Expected value (e) Deviation² (d²) d²/e Deviation…les/test/tq.php?testid=2804&strandid%3D&element%3D&difficulty=assessment&assignment_id%3D45196931&load_test%-D1&teacherPr B Brainly.com-For st.. O Instagram N Netflix a Escape Room (Und. USATestprep, LLC - E Google Docs Stu wrerhd.cells at rer rote due to the siiple diffsion of Save C) 01ann suan an sianua LUPInaPLiaipaih ep snan aun aAPa IM JaPAA active transport. Glucose molecules will move from the cells into the solution in the beaker D) through facilitated diffusion. When examining the major macromolecules in the cell, a student isolates two molecule types that are directly responsible for cellular energy. A comparison of which molecule types will be most likely to include the molecules primarily responsible for cellular energy? A) proteins and lipids B) carbohydrates and lipids proteins and carbohydrates D) carbohydrates and nucleic acids Proteins and polysaccharides are polymers. These polymers are formed by dehydration synthesis. Which statement correctly identifies a…
- Please count the cells in grids 1, 3, 7, and 9. Calculate the cell density (a) and cell viability (b) for this sample. (assume we started with 100 microliters of cells and added 100 microliters of trypan viability blue). Hemacytometer Grid 123 5 6 789 4 Enlarged view O O O 8Match the following experimental methods with their specialty procedures and objectives. autoradiography use of green fluorescent protein (GFP) fractional centrifugation use of genetic mutants A. silencing genes means inhibition of the expression of a protein B. fluorescing GFP protein is fused with a protein tracked in the cell C. biochemical processes are visualized using radiolabeled materials D. tissue samples homogenized at varying centrifugal force leads to isolation of desired cellular fractionHorizontal sequence :VIRL Vertical sequence:MKF Scoring rules: g/o = -3, g/e = -1, match or mismatch - from PAM250 substitution matrix below. SW algorithm. 1. Complete the scoring matrix. Scoring matrix with PAM250 scores: V I R L M K F 2. Set up, initialize and complete the SW matrix. 3. Retrace, align and score alignment(s). Use the arrows and circles for the matrix and path(s). V I R L M K F Align and score all optimal alignments here.
- Horizontal sequence :VIRL Vertical sequence:MKF Scoring rules: g/o = -3, g/e = -1, match or mismatch - from PAM250 substitution matrix below. SW algorithm. 1. Complete the scoring matrix. Scoring matrix with PAM250 scores: V I R L M K F 2. Set up, initialize and complete the SW matrix. 3. Retrace, align and score alignment(s). Use the arrows and circles for the matrix and path(s). V I R L M K F Align and score all optimal alignments here. PLZ the arrows and circles for the matrix and path(s) AND SHOW ALL possible AlignmentWe need to prepare a stock solution of medium for your culture cells, which usually includes liquid salt solution and bovine serum. Our liquid salt solution is supplied in a 50X concentration, and we need to dilute it to 1X for use. We also need to add 75% fetal bovine serum for a final concentration of 15%. How would we make up 0.80 liters of this culture media using water as our solvent?1. What are the advantages and disadvantages of viable cell counts and turbidimetric method? Discuss it comprehensively and please, do not jus copy from somewhere.