1. You are determining the effect of pH in the metabolism of pyruvate. The pH meter you used gave an erroneous reading of pH 8 instead of the actual pH of the 0.5 M phosphate buffer equal to 10.5. What would be the possible outcome? Explain why. a. More precipitate will be observed. b. Less precipitate will be observed. c. No precipitate will be observed at all. d. Insufficient information 2. The metabolism of a new species of bacteria obtained from the depths of the sea was observed in the lab using glucose and methylene blue. The results after incubation of the bacteria at different temperatures are tabulated below. Temperature Observation 10°C Dark blue 35°C Dark blue 95°C Light blue Which of the following is most likely true? Why? a. The bacteria will still actively metabolize upon storage in cold temperatures. b. The bacteria is thermophilic. c. Heat is possibly an inhibitor in its TCA cycle. d. A and C
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
1. You are determining the effect of pH in the
a. More precipitate will be observed.
b. Less precipitate will be observed.
c. No precipitate will be observed at all.
d. Insufficient information
2.
The metabolism of a new species of bacteria obtained from the depths of the sea was observed in the lab using glucose and methylene blue. The results after incubation of the bacteria at different temperatures are tabulated below.
|
Temperature |
Observation |
|
10°C |
Dark blue |
|
35°C |
Dark blue |
|
95°C |
Light blue |
Which of the following is most likely true? Why?
a. The bacteria will still actively metabolize upon storage in cold temperatures.
b. The bacteria is thermophilic.
c. Heat is possibly an inhibitor in its TCA cycle.
d. A and C
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