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- 19. Which of the following statements about actin is true? A. Binding of actin filaments by ARPS promotes disassembly of actin filaments. B. Actin treadmilling occurs at high concentrations of actin. C. Focal adhesions are released at the beginning of cell movement and do not reform until the cell has reached its destination. D. Actin polymerization initiates protrusion of the membrane in lamellipodia and filopodia.Which of the following do not play a role in intracellularmovement?a. microfilaments and intermediate filamentsb. microfilaments and microtubulesc. intermediate filaments and microtubulesd. only intermediate filamentsA melanosome (type of vesicle containing the pigment melanin) is transported towards the nucleus of the cell. The hydrolysis of ATP is involved with this movement. Identify the most likely motor protein and cytoskeletal filament involved with this transport. a. Myosin, microtubules b. Kinesin, actin filaments c. Dynein, actin filaments d. Dynein, microtubules e. Kinesin, microtubules f. Myosin, actin filaments
- 11. Following figure shows the leading edge of a lamellipodium. Which of the following statements is false. cell actin leading edge monomers capping protein A. Nucleation of new filaments near the leading edge pushes the plasma membrane forward. B. ARP proteins nucleate the branched actin filaments in the lamellipodium. C. Capping proteins bind to the minus end of actin filaments. D. There is more ATP-bound actin at the leading edge than in the actin filaments away from the leading edge. 12. Which of the statements below about intermediate filaments is false. A. They can stay intact in cells treated with concentrated salt solutions. B. They can be found in the cytoplasm and the nucleus. C. They can be anchored to the plasma membrane at a cell-cell junction D. Each filament is about 10 um in diameter.1.There are different processes for transport of molecules of ions across cell membranes. Explain briefly each process a. Simples diffusion b. Facilitated diffusion via carrier proteins c. Facilitated diffusion via ion channels d. Primary active transport e. Secondary active transport f. Endocvtosis g. Exocytosis h. Transcytosis 2. List four Physical factors that affect the rate of diffusion and how they will affect it. 3. What kind of molecules can pass through the plasma membrane of the cells through simple diffusion? Give brief discussion. 4. List the three main parts of a cell and explain their functions. 5. Define an organelle. Which organelles are surrounded by a membrane and which are not? 6. What is the key difference between passive and active process? 7. Why RNA transcription can still serve the needs of the cell even if its operation is slow compared to DNA?1.Cytochalasin is a drug that caps actin filament plus ends, thus preventing actin polymerization. Phalloidin is a drug that binds to and stabilizes actin filaments, preventing actin depolymerization. Even though these drugs have opposite effects on actin polymerization, the addition of either of these drugs instantaneously freezes the cell movements that depend on actin filaments. Explain why drugs that have opposite effects on actin filaments can have a similar effect on cell movements.
- The tigroid lysis is observed in a case of functional exhaustion of the neuron. It is resulted in decay of basophilic substance and brightening of the neuroplasma. What organelles changes do this phenomenon is associated with? a. Granular endoplasmic reticulum O b. Lysosomes O c. Neurofibrils d. Golgi apparatus O e. Mitochondria1. Which of the listed protein would NOT be expected to have experienced vesicular transport? A. Cis-golgi enzymes B. Proteins in early and late and endosomes due to pinocytosis C. Lysosomal enzymes D. A multi-pass transmembrane protein in the plasma membrane E. All of the above proteins Actually would undergo vesicular transport at some point 2. What would be the effect of COPI-coated vesicles if a cell is treated with GTP analogous that can bind the ARF G protein but can not trigger the exposure of its lipid anchor. A. They will decrease substantially in number in the cytoplasm B. They will accumulate in the nucleus C. They will fuse into one giant vesicle that was seen in the cytoplasm D. They will accumulate in the cytoplasm E. They will decrease substantially in number in the nucleusWhat sort of cellular changes would you expect a sailor sick with scurvy to experience? O Their collagen (an extracellular matrix component) would be diminished, resulting in fewer binding sites for integrins. The end result would be less adherent cells. O Their collagen (a cytoskeletal fiber) would be diminished, resulting in a loss of contractile motion within the cell, affecting both cell migration and muscle function.
- 1. Name the two types of structures that mediate cell-cell contact and are associated with intermediate filaments. 2. The dynamic behavior of microtubules is regulated by a variety of MAPs. What does this stand for? 3. Name the two motor proteins responsible for powering the variety of movements1. Phosphodiesterase directly functions to a. enzymatically activate the alpha subunit of the G protein b. enzymatically cleave a membrane phospholipid to form IP3 and DAG c. enzymatically inactivate cyclic GMP d. open Ca++ channels in the sarcoplasmic reticulum e. none of the above 2. The molecule on the picture marked (*) is a (mark all apply) a. G protein-coupled hormone receptor b. second messenger c. protein kinase enzymeMatch each term to the phrase that describes it best. Cell fiber that can be found in muscle cells a. Primary cilium e. vLargest of the cell fibers; act like "engines" for the cell b. Microfilaments C. Gap junctions d. Centrosomes e. Microtubules f. Cilia g. Intermediate filaments h. Microvilli i. Flagella j. Desmosomes k. Centrioles 1. Tight junctions