1. What important by‐products are obtained in commercial fermentation processes? 2. Of what value is iodoform test? 3. What is meant by each of the following? a. denatured b. absolute c. proof
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1. What important by‐products are obtained in commercial fermentation processes?
2. Of what value is iodoform test?
3. What is meant by each of the following? a. denatured b. absolute c. proof
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- 1. MEDIA. Match the name of the medium with the physiological test. A medium may be used more than once. Tests may require more than one answer.a. Kligler’s iron agar 2,3-butanediol fermentationb. MR-VP broth carbohydrate fermentationc. phenol red lactose casein hydrolysisd. SIM medium citrate utilizatione. Simmon’s citratr hydrogen sulfide productionf. skim milk agar mixed-acid fermentationg. spirit blue agar triglyceride hydrolysish. tryptone broth tryptophan degradationAn autoclave is typically used for sterilization of media. a. Define sterilization. b. Under what conditions are media typically sterilized in an autoclave? c. What type of media components cannot be sterilized in an autoclave? d. What is an alternative to autoclaving for sterilizing heat-sensitive materials?1. In an autoclave, how are temperatures above boiling achieved?(short answer) 2.. Can culture media be sterilized in the hot air sterilizer? Why or why not? (short answer) 3.What is the hazard of the spattering tendency in heating a wire loop with specimens over a flame? (short answer)
- Agar is a solidifying agent used in media preparation.a. What is its origin? b. What makes it ideal for cultivation of microbes? c. How and why does the agar concentration in semisolid media differ from conventional solid media?1. What results are expected if you drop H2O2 into a cut on your arm? Explain why those results are expected. 2. To do this test, why do you have to grow the organism in medium containing tryptophan? 3. Why is a red color after the addition of zinc a negative result for nitrate reductase?2. Which of the following statements is true regarding steam sterilization? a. It is a universal technique, applicable to a product of any nature. b. At the same temperature, usually more effective than dry heat. c. Steam sterilization is amenable to a continuous workflow. d. The purpose of the steam is to keep the product from drying out during sterilization. Steam cannot be combined any other sterilization technique. е.
- If you streak a sample from your nose onto a Mannitol salt agar plate and the medium turns yellow, what does that tell you? (choose all that apply) a. The organism is a yeast b. The organism ferments mannitol c. The organism is beta-hemolytic d. The organism is likely Staphylococus aureus e. The organism is acid tolerantIn the tripple sugar fermentation test, why is the triple sugar iron agar called a multi-test system? list the concentrations of sugars in the media and the possible results due to fermentationExplain the mechanism leading to the given reactions in the inoculated biochemical media. 1. Blackening of the medium in Triple Sugar Iron Test slant2. Development of pink to red color in nitrate broth in Nitrate Reduction Test
- 1. Given the following common laboratory materials, which of the three methods of heat sterilization do you think is the most appropriate and practical to use in sterilizing them Why do you think so ? Method of heat sterilization Reason a. Empty flasks b. L-rod (glass rod) c. 10 ml glass pipet d. Wire needles e. Antibiotic solutionWhat is the best method to filter the precipitate from it's reaction mixture? a Gravity filtering is accomplished by folding the filter paper into a cone and placing it in a long stem funnel. b Vacuum filtering using a Buchner funnel with filter paper. c Using a filtering crucible that can be dried and ignited at red heat. d All of the above, just depends on the precipitate.1. Give the importance of carbohydrate fermentation test in biochemical testing and enumerate the enzymes that are involved. (5 points) 2. What are the substances added in the culture media utilized by the organism producing hydrogen sulfide? Give 2 other media used for the production of hydrogen sulfide. (5 points) 3. Explain the mechanism taking place in the hydrolysis of urea which leads to the formation of bluish red color. ( 5 points) 4. Discuss why human blood plasma will not always yield reliable results. (5 points)
