1. DNA can be cut at specific sequence motifs using enzymes called restriction endonucleases. You obtain a single PCR product from R. sphaeroides DNA that can be cut in the places shown below by solid arrows. These are the Clal specific restriction motifs. Positions of these restriction sites are indicated by nucleotide numbers in a 1059 nucleotide DNA fragment. Draw the expected band(s) for each of the bacterial species on the gel picture below if treated with the restriction enzymes as illustrated. Rhodobacter sphaeroides 2. 4.1 1059 0 290 749 Rhodopseudomonas palustris BisB5 0 Dinoroseobacter shibae DFL 12 0 290 Silicibacter sp. TM 1040 0 1059 749 1059 1059 DNA ladder R. sphaeroides 2. 4. 1 D. shibae DFL12 R. palustris BisB5 Silicibacter sp. TM1040 1100 1000 900 800 700 600 500 400 300 200 100 |||||||| CS Scanned with CamScanner 54

You learned in the chapter that an STR locus is a locus where alleles differ in the number of copies of a short, tandemly repeated DNA sequence. PCR is used to determine the number of alleles present, as shown by the size of the DNA fragment amplified. In the Figure below are the results of PCR analysis for STR alleles at a locus where the repeat unit length is 9 bp, and alleles are known that have 5 to 11 copies of the repeat. Given the STR alleles present in the adults, state whether each of the four juveniles could or could not be an off-spring of those two adults. Explain your answers.

Transcribe and translate the following DNA sequence (nontemplate strand): 5’-ATGGCCGGTTATTAAGCA-3’

Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning vectors? a. The plasmid must have resistance to accept DNA inserts. b. They allow the detection of plasmids that contain an inserted DNA fragment. c. They ensure the presence of the ori site. d. They ensure that the plasmid can be cut by a restriction enzyme. e. They allow identification of bacteria that have taken up a plasmid.