1. As has been discussed in class the electron transport chain is the first part of oxidative phosphorylation. This chain of multi-subunit enzymes is responsible for facilitating the transfer of electrons from NADH and succinate/FADH₂ to oxygen making water. Given this understanding answer the following questions. a. Below is an equation for determining the amount of energy required to move 1 mol of protons across the inner membrane of the mitochondria. In actively respiring mitochondria the A = +/-0.15 volts to +/- 0.20 volts. The ApH for a typically mitochondria will be 0.75 pH units, with the matrix (N-side) being more alkaline than the IMS (P-side). Given this state the total energy needed to move a single proton from the N to the P-side. Assume T = 310K and R = 8.314 J mol-¹ K-¹ and a A4 = 0.17 volts. Finally, assume F = 96.5 kJ/mol/volt AG = 2.3RTAPH + FA b. How much free energy is available in the transfer of electrons from NADH to oxygen given the following half-reactions and free energy equation. Assume F = 96.5 kJ/mol/volt. AG¹° = -nFAE¹0 ΔΕΙΟΞΕΙΟ (electron acceptor) - E'o (electron donor) ½2 O₂ + 2H+ + 2e →→ H₂O E'o = 0.816 volts NAD+ + 2H+ + 2e → NADH + H+ E' = -0.320 volts C. How much free energy is available in the transfer of electrons from FADH₂ to oxygen given the following half-reactions and free energy equation. Assume F = 96.5 kJ/mol/volt. AG'O-nFAE¹⁰ AE¹0 = E¹0 (electron acceptor) - E¹ (electron donor) ½ O₂ + 2H+ + 2e → H₂O E¹° 0.816 volts FAD + 2H+ + 2e → FADH₂ E¹0 = -0.219 volts d. Given your answers in parts a.- c. calculate the percent of the energy from each redox reaction that is stored within the proton gradient for both NADH and FADH2
1. As has been discussed in class the electron transport chain is the first part of oxidative phosphorylation. This chain of multi-subunit enzymes is responsible for facilitating the transfer of electrons from NADH and succinate/FADH₂ to oxygen making water. Given this understanding answer the following questions. a. Below is an equation for determining the amount of energy required to move 1 mol of protons across the inner membrane of the mitochondria. In actively respiring mitochondria the A = +/-0.15 volts to +/- 0.20 volts. The ApH for a typically mitochondria will be 0.75 pH units, with the matrix (N-side) being more alkaline than the IMS (P-side). Given this state the total energy needed to move a single proton from the N to the P-side. Assume T = 310K and R = 8.314 J mol-¹ K-¹ and a A4 = 0.17 volts. Finally, assume F = 96.5 kJ/mol/volt AG = 2.3RTAPH + FA b. How much free energy is available in the transfer of electrons from NADH to oxygen given the following half-reactions and free energy equation. Assume F = 96.5 kJ/mol/volt. AG¹° = -nFAE¹0 ΔΕΙΟΞΕΙΟ (electron acceptor) - E'o (electron donor) ½2 O₂ + 2H+ + 2e →→ H₂O E'o = 0.816 volts NAD+ + 2H+ + 2e → NADH + H+ E' = -0.320 volts C. How much free energy is available in the transfer of electrons from FADH₂ to oxygen given the following half-reactions and free energy equation. Assume F = 96.5 kJ/mol/volt. AG'O-nFAE¹⁰ AE¹0 = E¹0 (electron acceptor) - E¹ (electron donor) ½ O₂ + 2H+ + 2e → H₂O E¹° 0.816 volts FAD + 2H+ + 2e → FADH₂ E¹0 = -0.219 volts d. Given your answers in parts a.- c. calculate the percent of the energy from each redox reaction that is stored within the proton gradient for both NADH and FADH2
Human Physiology: From Cells to Systems (MindTap Course List)
9th Edition
ISBN:9781285866932
Author:Lauralee Sherwood
Publisher:Lauralee Sherwood
Chapter2: Cell Physiology
Section: Chapter Questions
Problem 2TAHL
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Transcribed Image Text:1. As has been discussed in class the electron transport chain is the first part of oxidative
phosphorylation. This chain of multi-subunit enzymes is responsible for facilitating the transfer of
electrons from NADH and succinate/FADH₂ to oxygen making water. Given this understanding
answer the following questions.
a. Below is an equation for determining the amount of energy required to move 1 mol of
protons across the inner membrane of the mitochondria. In actively respiring mitochondria
the A = +/-0.15 volts to +/- 0.20 volts. The ApH for a typically mitochondria will be 0.75 pH
units, with the matrix (N-side) being more alkaline than the IMS (P-side). Given this state
the total energy needed to move a single proton from the N to the P-side. Assume T = 310K
and R = 8.314 J mol-¹ K-¹ and a A4 = 0.17 volts. Finally, assume F = 96.5 kJ/mol/volt
AG = 2.3RTAPH + FA
b. How much free energy is available in the transfer of electrons from NADH to oxygen given
the following half-reactions and free energy equation. Assume F = 96.5 kJ/mol/volt.
AG¹° = -nFAE¹0
ΔΕΙΟΞΕΙΟ (electron acceptor) - E'o
(electron donor)
½2 O₂ + 2H+ + 2e →→ H₂O E¹° 0.816 volts
NAD+ + 2H+ + 2e → NADH + H+ E' = -0.320 volts
C. How much free energy is available in the transfer of electrons from FADH₂ to oxygen given
the following half-reactions and free energy equation. Assume F = 96.5 kJ/mol/volt.
AG'O-nFAE¹⁰
AE¹0 = E¹0 (electron acceptor) - E¹O (electron donor)
½ O₂ + 2H+ + 2e → H₂O E'O = 0.816 volts
FAD + 2H+ + 2e → FADH₂ E¹0 = -0.219 volts
d. Given your answers in parts a.- c. calculate the percent of the energy from each redox
reaction that is stored within the proton gradient for both NADH and FADH2
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