1. a) What are Romanowsky stains? Name four of these. b: What are the components of a Romanowsky stain? b: What is the optimum pH for staining with Giemsa. c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.

Q: Explain the reasons you would use a negative stain?

A: In a negative stain the slide is actually stained. This is because the negatively charged stain…

Q: Is it possible to perform double or triple staining using fluorescent dyes? Explain

A: For both membrane and soluble proteins, fluorescent dyes are increasingly being utilized to monitor…

Q: What kind of stain would a pathologist use to visualise a chrondrosarcoma under the microscope? why…

A: Staining is a method for enhancing contrast in material, usually on a tiny scale. Biological…

Q: You receive a bacterial culture in the lab and your job is to figure out information which will be…

A: Best option to stain a bacterial culture is Gram Stain Method. It is used to determine gram status…

Q: Explain the mechanism of staining for each of the stains used. Iodine Methyl green Nigrosin

A: The living cells can be seen under a microscope. The cells is not visible properly as it is…

Q: Why are basic dyes more effective for bacterial staining than acidic dyes?

A: Staining is a technique used in slide preparation to colour the specimen.      With light microscopy…

Q: Why is the wavelength of the light that is released in spectrophotometric analysis longer than the…

A: Some molecules absorb light at a particular wavelength and emit light in higher wavelength and this…

Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…

A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…

Q: What are the principle and basic concepts of DIFFERENTIAL staining?

A: The simple dye is used in the technique of simple staining to highlight only the specific structures…

Q: Is heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain…

A: In capsule staining procedure “we do not heat fix and rinse the smear with water” as heat and water…

Q: Why must heat or a surface-active agent be used with application of the primary stain during…

A: During acid fast staining first of all heat is used to fix the bacterial smear onto the slide.…

Q: What color would gram positive cells appear if ghr safranin staining step was left out of staining…

A: Gram staining is a technique used for differentiating the bacteria based on their cell wall…

Q: What is the difference between simple stain and complex stain? Describe the color of an S. aureus…

A: Most types of cells don't have a normal pigment and are in this way challenging to see under the…

Q: What is the purpose of acid-fast staining? What information can you gain from this procedure? Please…

A: The acid-fast stain is used to identify acid-fast organisms. Acid-fast organisms posess nearly…

Q: Which step is the most crucial or most likely to cause poor results in the Gram stain?

A: Gram staining is a common technique that is used to differentiate bacteria into two broad groups…

Q: Let's assume that you were teaching the Gram stain to four students, and each student did one thing…

A: Gram staining may be a staining strategy utilized to distinguish and recognize bacterial living…

Q: What is a differential stain? why is it used?

A: Differential stain is a type of stain use to identify organisms.

Q: What is the principle of Giemsa staining and  components of Giemsa stain?

A: The cells are the basic structural and fundamental unit of the living system. It consists of…

Q: Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can…

A: According to the guidelines we have to answer first 2-3 question rest you can ask separately thank…

Q: What are the principle and basic concepts of NEGATIVE staining?

A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…

Q: What are the most commonly used methods in staining? why?

A: Introduction  Staining is a technique for enhancing contrast in material, usually on a tiny scale.…

Q: How are negative stains prepared?Name two applications for which this type of stain especially…

A: Negative staining is defined as a method of demonstrating the type of small objects (like bacteria)…

Q: Describe how simple staining and fluorescence staining are similar and how they are different. What…

A: Microscopy is a technical field for the use of microscopes to visualize objects and locations of…

Q: Why is a blank used in the spectrophotometer before the actual pigment is tested?

A: spectrophotometer is a device used in spectral analytical techniques like UV spectroscopy , in which…

Q: Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?

A: In microscopic studies, the specimen needs to be prepared for an optimized vision under a…

Q: What is the advantage of the Kinyoun staining procedure over the Ziehl-Neelsen method?

A: Kinyoun staining is a technique used to stain bacteria. Specifically, acid-fast species of the…

Q: Explain the difference in the working principles of Dark-field and Light-field microscopes using pKk…

A: Microscope: It is an optical instrument that uses a lens or a combination of lenses to produce…

Q: What is an example of a negative stain?

A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…

Q: Briefly discuss the difference between simple and differential staining? Which is better suited for…

A: Staining is a laboratory technique in which various coloured dyes are used to highlight the specimen…

Q: What are the principle and basic concepts of Simple staining

A: The simple staining doesn't give a lot of data about the cell separated from the bacteria'…

Q: a.) Give the purpose of heat fixation. b.) What can be observed in wet mounts or hanging-drop slides…

A: The Purpose of fixation of a smear is as follows: 1) Tissue Killing- As this will help to prevent…

Q: Give three situations where the negative staining procedure would be used.

A: Negative staining is a specimen preparation technique that requires an acidic dye such as India Ink…

Q: Use the table to differentiate between agarose gel electrophoresis and SDS-PAGE based on the…

A: Agarose Gel Electrophoresis is the technique of separating DNA or protein. SDS-PAGE is the technique…

Q: What is the difference between Giemsa and Wright stain?

A: Staining is a technique used to enhance contrast in samples, generally at the microscopic level.…

Q: In negative staining, the acidic stain will penetrate the cell. In negative staining, it will create…

A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…

Q: What is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain,…

A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…

Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…

A: The staining is done to enhance the visualization of cell and certain cellular component under a…

Q: What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What…

A: In microbiology, the technique that is used to enhance the contrast in samples, at the microscopic…

Q: Which of the following is/are true regarding the acid-fast stain? (There may be more than one…

A: Answer  First option  Non-acid-fast microbes appear blue in a completed acid-fast stain.

Q: 5. You receive a bacterial culture in the lab and your job is to figure out information which will…

A: Answer One of the most important staining methods used to differentiate between Gram Positiva and…

Q: k there are 2 staining reagents applied in differential s

A: Differential Staining-  It is a staining process which uses more than one staining reagent.  it is…

Q: For What purpose  is Giemsa stain used for?

A: Staining is a technique used in microscopy. Staining is mainly used in biology to highlight the…

Q: In acid fast staining, lipoidal material of acid fast cells absorbs the methylene blue. Upon the…

A:

Q: What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary…

A: Need to explain the primary stain in the Kinyoun acid-fast stain.

Q: For each stain, indicate if it’s a cationic dye or an anionic dye (2 pts).             Stain…

A: Cationic Dyes- These are the dyes which dissociates into cations in aqueous solutions. Anionic Dyes-…

Q: What is the effect when xylene is not removed when staining?  What is/ are the specific action/s of…

A: Staining is a procedure in which the coloured dye or a stain is added to a specimen so that it may…

Q: .) What is the purpose of acid-fast staining? What information can you gain from this procedure?…

A: Acid fasr staining is the differential staining method which was first developed by Ziehl and…

Q: Name two conditions, which will affect the quality of your staining. B.What is a panoptic stain?…

A: Staining is a very important part in Histology, without which it would be impossible to study the…

Q: What is the difference between simple and differential stains? Give an example of a differential…

A: Bacteria are microorganisms that are colorless for most of the part and in order to study their…