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1. What are the other three different types of microtome (except hand microtome). Describe and differentiate the three.
2. In preparing a slide, what is the significance of heating the sample after it is stained?
3. Give at least two other examples of samples that are best prepared using Smear Preparation
Technique.
Step by step
Solved in 2 steps
- 1. Why must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?2. Explain the importance of using methylene blue as the counterstain in the acid-fast staining method. 3. What is the specific diagnostic value of this staining procedure?4. What is the specific diagnostic value of this staining procedure?5. Explain the importance of using methylene blue as the counterstain in the acid-fast staining method.6. What is the specific diagnostic value of acid fast staining procedure?1. What is the usual stain used for staining a sputum smear? 2. Give the principle of acid fast staining and gram staining. 3. Explain the Bethesda System. 4. How will you instruct a patient that will undergo a brochoscopy?1. What is the purpose for using stains? What microbial characteristics can one ascertain from a simple stain? 2. Why is it necessary to make a heat-fix smear and what are the disadvantage of heat fixing?
- 2. Give the purpose of each of the following reagents in a differential staining procedure: (Primary stain, Counter stain, Decolorizing agent and Mordant)1. What is the significance of soaking the container in bleach? 2. What is the significance of keping the container closed except when pouring the medium; partially opening the container during pour-plating in preparing culture media?1. What is the usual stain used for staining a sputum smear? 2. Give the principle of acid fast staining and gram staining.
- 1. Define Differential Staining. 2. Application of differential staining technique.Cite the purpose of each of the following reagents in a differential stainingprocedure.a. Primary stain:b. Mordant:Lab reportExPEriMEnt9 90 Experiment 9: lab reportc. Decolorizing agent:d. Counterstain:1. What is the purpose for using stains? What microbial characteristics can one ascertain from a simple stain? 2. Why is it necessary to make a heat-fix smear and what are the disadvantages of heat fixing? 3. what is the best age for your culture when performing a gram stain? Why? 4. Why is Gean staining considered a differential staining process? 5. What are some reasons a Gram positive cell might appear Gram negative?
- Microtome 1. Define microtome instrument 2. Mention the types of microtome instrument 3. What are the principle operation of any type of microtome? 4. Compare between two typo of microtome difference from principle operation1.Procedure for sectioning paraffin blocks 2.Mann's methyl blue eosin stain?1.)What is the purpose of a counterstain? 2. What does a mordant do in the Gram stain procedure? Which reagent in the Gram stain is the mordant? 3. True or False? The oil objective should make contact with the oil on the slide. 4. Why is it necessary to let bacterial smears completely air dry before heat fixing? 5.Why should controls be included wherever possible for any staining technique? 6. Why is it necessary to heat the slides while staining for endospores?