. Describe and list the steps involved in DNA replication. 2.

1... Describe the different models for DNA replication (conservative, semi-conservative, dispersive) and the experts that demonstrated which approach is actually used in cells

Describe several aspects about RNA polymerases that are unique compared to DNA polymerases.

1. Identify the three protein complexes/enzymes important in eukaryotic DNA replication A. Enhancer, silencer, promoter  B. DNA polymerase, RNA polymerase, mediator C. Start codon, stop codon, RNA polymerase D. Prereplication complex, origin recognition complex, MCM helicase

In bacterial RNA polymerase, the sigma factor A. contains the catalytic activity of the polymerase  B. remains part of the polymerase throughout transciption. C. recognizes promoter sites in the DNA  D. recognizes transcription termination sites in the DNA E. is a subunit of DNA polymerase III

1.) Compare and contrast DNA polymerase and RNA polymerase? 2.) Compare and contrast transcription in prokaryotes vs. eukaryotes?

D. radiodurans can survive strong ionizing radiation (gamma radiation). What DNA repair process? a. Reverse transcription b. Translation c. Homologous recombination d. DNA replication e. Transcription

2

1. Determine the effect of the following mutations on the DNA sequence. In each case, the mutation is described after the sequence (REFER TO THE SUPPLEMENTAL DOCUMENT FOR GUIDANCE TO THIS QUESTION). Adenine nucleotide (A shown in red below) was inserted into the DNA sequence at the position indicated by the arrow). Write out the sequence of the mutated DNA and the protein made from it. What is the effect of this mutation on the protein? (For example, how will the mutation affect the length and sequence of the protein? What about the function of the protein?) A 3' TACATGG'TTGTGCTAATT 5'

1.What is DNA replication? 2. What are the principles of DNA replication?