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Cellular Respiration Lab Report

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What is cell respiration? Well cell respiration refers to the procedure where the chemical energy of organic molecules is changed into a type that can be used by organisms. It derives energy from what we eat or from our fuel, more specifically from glucose. Meaning that cell respiration consumes oxygen and organic molecules such as glucose. Therefore glucose is oxidized and oxygen is reduced. Cell respiration is the most prevalent and efficient catabolic pathway. It require transfusions of energy from outside sources to perform their many tasks. The the objective of this experiment was to measure the concentration of oxygen gas and carbon dioxide gas. Also to determine whether germinating peas respire. The hypothesis would be that germinating …show more content…

Then, setup up your CO2 gas sensor to the low (0-10,000ppm) setting. After that you will connect the O2 gas sensor to channel one and the CO2 gas sensor to channel two to the lab quest. Next, choose new from the file menu. With a thermometer measure the room temperature and record it. Now, gather 35 germinated peas and set them dry with a piece of paper. Then, put the O2 gas sensor into the bio chamber 250 and the CO2 gas sensor into the neck of the bio chamber 250. Wait two minutes and start collecting data by pressing the play button located at the bottom left. Next, after data has been collected, remove the peas and place them in a 100 Ml beaker filled with cold water and ice cubes. For every run performed you will rinse the respiration chamber with water and dry the inside with paper towel. Also for every run you will perform a linear regression to calculate the rate of respiration. For this you will need to go to analyze menu and choose curve fit and select CO2 gas. Then select linear as the fit equation. After that you will enter the absolute value of the slope and press ok. Repeat the procedures used above for run two and three. For run three you will grab and dry the peas that were placed in cold water. When done disconnect the sensors. Next, save all three runs with a name that you will remember. Print all runs by connecting the lab quest to a printer using a USB

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