Oxygen Gas Lab Report

Peroxidase is an enzyme found in potatoes that catalyzes the breakdown of hydrogen peroxide, H2O2, into O2 gas and water. We examined the different pH environments that can affect the enzyme activity during the breakdown of H2O2. In order to do this, we added different levels of pH, low, medium, and high, into different test tubes with the enzyme and H2O2, and we then inverted the tube. The amount of O2 gas produced was then measured and recorded. The result was that the higher pH produced more gas, followed by medium pH, then low pH. The enzymes were more active in the pH of about 10. It increased

However, because the deviation between the oxygen and ideal gas behaviors are very small in the experiment environment and thus can be ignored. So, the ideal gas equation PV=nRT can be used to determine the mole of oxygen generated. Equipment & Reagents Equipment: graduated flask, test tube, alcohol burner, pipette, rubber catheter, glass catheter, glass flume, iron support, dispensing spoon, match, balance, weighing paper. Reagents: KClO_3, MnO_2; (Potassium chlorate, Manganese

The purpose of this experiment was to simply measure oxygen production rates released from decomposed hydrogen peroxide under different conditions (concentration of enzymes, temperature, and PH level).

The experiment first started by the calculation of the net oxygen production under white light, it started by putting the Elodea in a volumeter tube containing 3% sodium bicarbonate solution, which was assembled to the volumeter by covering the tube with the plug/pipet/syringe assembly (a 1-2cm air space between the bicarbonate solution and the top rubber cover of the volumeter tube should be left.) Then, clamp the volumeter onto the clamp stand using the tube clamp and fill 2000ml beaker with cold tap water. The beaker should be placed on the clamp stand between the volumeter tube and the light lamp. A five minutes stabilization

Abstract: This lab’s purpose was to see how different levels of yeast, distilled water, and sugar interact to affect the level of carbon dioxide evolved in fermentation. In this experiment we had two sections. The first section tested four test tubes with varying levels of yeast, glucose and distilled water for evolved carbon dioxide levels. The tubes were timed for 20 minutes. The amounts of solution in the test tubes are noted in the methods section of this lab report. The second section of the lab used three test tubes and flowed the same procedure except added spices. The levels of ingredients are also in the methods section. The main goal of this experiment was to see the effects of yeast concentration.

The leveling bulb must be leveled with the amount of water in the buret at all times. Timing should begin after 2 ml of oxygen has been produced and must be recorded at 2 ml intervals until 14 ml of oxygen has accumulated. After recording the time, the first part of the experiment is now complete.

PH can affect the way fermentation occurs due to the irregularity of the acidity or alkalinity within the glucose solution. This is an enzyme-based reaction that is susceptible to pH. The aim of this experiment was to determine how pH affects the yeast fermentation rate by performing the experiment numerous times with a different pH of glucose solution which included pH 3, 5, 7, 9, 11. The hypothesis was ‘If the pH is lower than the neutral point then the fermentation reaction will occur faster?’ The experiment conducted was to measure the amount of C02 produced by the yeast going into fermentation, however varying the pH of glucose solution by using different pHs . To test this every 5 minutes the volume of gas in the test tube was observed and recorded until a period of 30 minutes had been. The end results

This experiment is designed to analyze how the enzyme catalase activity is affected by the pH levels. The experiment has also been designed to outline all of the directions and the ways by which the observation can be made clearly and accurately. Yeast, will be used as the enzyme and hydrogen peroxide will be used as a substrate. This experiment will be used to determine the effects of the concentration of the hydrogen peroxide versus the rate of reaction of the enzyme catalase.

Testing the Effects of Temperature on the Decomposition of Hydrogen Peroxide with the Enzyme Catalase

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Hypothesis: If the mass of yeast (g) is increased the rate of fermentation of glucose (mL/s) will increase.

Compute the uncollectible account expense, and make the appropriate journal entry, for the current year assuming the uncollectible account expense is determined as follows:

Next, fifteen drops of potato juice were added to each test tube. Simultaneously, ten drops of Hydrogen Peroxide (H2O2) were added in all test tubes at the same time. All test tubes were gently shaken to mix the solutions and put to rest. After three minutes, the height of the bubbles of the three test tubes were measured at the same time. Then, the test tubes were exposed to different temperatures.

The procedure for this experiment was to first obtain four balloons and blow them up in order to stretch them. Then obtain and fill the four large test tubes each with thirty milliliters of warm forty degrees Celsius water and two grams of dry yeast which was weighed on a scale and scooped out by a spatula. After five milliliters of water, ten percent glucose, fructose or sucrose went into one of the four test tubes. Then parafilm was placed on top of each of the test tubes to seal them and they were swirled activating the yeast through rehydration. After swirling the film was removed and the balloons were tightly placed on the test tubes. Then finally observed the tubes build up of CO2 all the while swirling gently every fifteen minutes, recording observations.

7) The gas accumulation in the balloon was measured and recorded at one minute intervals for a total of 10 minutes (qualitative observations were included)