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E Chlorotica Lab Report

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This experiment performed by pierce have been a landmark paper in recent years which serve as the bases for many related studies. In this experiment , the specimen of E. chlorotica were collected from salt marsh located at Martha Vineyartd., being starved for more than 2 months and kept at 10 °C with fluorescent light under 14:10 light-dark illumination cycle [40]. The RNA were extracted from E. chlorotica and transported to BGI in Hong Kong for analysis. Researchers have also sequenced the genome of V. litorea. E. chlorotica transcriptom were aligned with V. litorea as a reference source and detect for region of similarity between sequences by BLAST. Once matching sequencies in V. litorea were detected , BlastX algorithm were applied …show more content…

litorea genome coding sequence. The matching genomic sequence with V.litoreea includes prk, pfk, tkt, lhc, thf1 they are genes that play a role in ‘photosynthesis ,carbon fixation , carbohydrate metabolism, thylakoid structure, chaperon activities and chloroplast maintenance processes ‘ [40]. There are also chloroplast-encoded transcripts that are important for the synthesis of chlorophyll a such as cyt F and RuBisCO and D1, D2 and CP43 that work in PSI and PSII center. While other proteins are having unidentified functions. These are important proteins which believed to sustain the kletoplast function.

The experimental result is of significance as the RNA of slug is extracted from whole sea slug extract that is free from algal contamination. The adult slug have been starved for 2 months where the digestive tract should have no remaining food residue while the larval used in the experiment have not been in contact with the algae [40]. Meanwhile the author explained that the integration of nuclear genes into slug genome could account for the low algal transcript level at near single

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