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Chloroplast Lab Report

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In this examination, four tubes of the chloroplast (Dark, 24cm, 30 cm, and 49 cm), buffer solution, water and DPIP were set in different strengths of light to decide how the light, powerful influences the reaction rate. The response rate was measured using absorbance values in five-minute increments. The tubes were put at 24 cm away, 30 cm away, 49 cm away, and totally dull. The tube nearest the light caused the smallest absorbance value, while the dim tube had the most astounding absorbance values. As the light power builds, the absorbance esteem and rate of the response diminished.
Introduction:
In this lab, DPIP was found as a substitute for NADPH in the electron transport chain to value the impact of illumination intensity on electron transport …show more content…

Each of the solutions and their respective blanks was put through a spectrometer at various wavelengths- increasing by increments of 50 nm each trial. The wavelengths of 400 nm to 500 nm had the best absorption rates.
Introduction:
The Four pigments: Carotene, Chlorophyll a, Xanthophyll, and Chlorophyll b are necessary when photosynthesis takes place in the stomata. The aim of this lab was to see how wavelength affected the rate of photosynthesis. My theory was the four pigments would have higher optical density with a shorter wavelength than the longer wavelength. While my null hypothesis was the different wavelengths will have no consequence on the observed levels of the four different pigments.
Methods:
Four pigments were procured from the technical assistant with blanks. The spectrophotometer was turned on to 400 nm. Once it was ready to use, the pigments were passed through to spectrophotometer one by one and recorded. Once the first trial was done, the spectrophotometer was increased to 450 nm. This process continued in 50 nm increments until the spectrophotometer is at 700 nm. After the 700-nm trial, the spectrophotometer can be turned

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