Insulin is a protein that allows sugar (glucose) to enter the body's cells (mainly liver, adipose tissue and skeletal muscle). This hormone plays a key role in the regulation of glucose levels in the blood ('hypoglycemic' effect). It is produced by the beta cells in the pancreas. A piece of the gene sequence that encodes for insulin contains: 5’ ATG-GCC-CTG-TGG-ATG-CGC-CTC-CTG-CCC-CTG-CTG-GCG-CTG-CTG-GCC-CTC-TGG-GGA-CCT-GAC- CCA-GCC-GCA-GCC-TTT-GTG-AAC-CAA-CAC-CTG-TGC-GGC-TCA-CAC-CTG-GTG-GAA-GCT-CTC-TAC-CTA-GTG-TGC-GGG-GAA-CGA-GGC-TTC-TTC-TAC-ACA-CCC-AAG-ACC-CGC-CGG-GAG-GCA-GAG-GAC-CTG-CAG-GTG-GGG-CAG-GTG-GAG-CTG-GGC-GGG-GGC-CCT-GGT-GCA-GGC-AGC-CTG-CAG-CCC-TTG-GCC-CTG-GAG-GGG-TCC-CTG-CAG-AAG-CGT-GGC-ATT-GTG-GAA-CAA-TGC-TGT-ACC-AGC-ATC-TGC-TCC-CTC-TAC-CAG-CTG-GAG-AAC-TAC-TGC-AAC-TAG 3’ a. Mutation of codon 34 (CAC to GAC) and 89 (CGT to CAT) of the insulin gene causes hyperinsulinemia which may contribute to diabetes. Identify the amino acid replacement from these mutations.
1. Insulin is a protein that allows sugar (glucose) to enter the body's cells (mainly liver, adipose tissue and skeletal muscle). This hormone plays a key role in the regulation of glucose levels in the blood ('hypoglycemic' effect). It is produced by the beta cells in the pancreas.
A piece of the gene sequence that encodes for insulin contains:
5’ ATG-GCC-CTG-TGG-ATG-CGC-CTC-CTG-CCC-CTG-CTG-GCG-CTG-CTG-GCC-CTC-TGG-GGA-CCT-GAC- CCA-GCC-GCA-GCC-TTT-GTG-AAC-CAA-CAC-CTG-TGC-GGC-TCA-CAC-CTG-GTG-GAA-GCT-CTC-TAC-CTA-GTG-TGC-GGG-GAA-CGA-GGC-TTC-TTC-TAC-ACA-CCC-AAG-ACC-CGC-CGG-GAG-GCA-GAG-GAC-CTG-CAG-GTG-GGG-CAG-GTG-GAG-CTG-GGC-GGG-GGC-CCT-GGT-GCA-GGC-AGC-CTG-CAG-CCC-TTG-GCC-CTG-GAG-GGG-TCC-CTG-CAG-AAG-CGT-GGC-ATT-GTG-GAA-CAA-TGC-TGT-ACC-AGC-ATC-TGC-TCC-CTC-TAC-CAG-CTG-GAG-AAC-TAC-TGC-AAC-TAG 3’
a. Mutation of codon 34 (CAC to GAC) and 89 (CGT to CAT) of the insulin gene causes hyperinsulinemia which may contribute to diabetes. Identify the amino acid replacement from these mutations.
2. Given is the 30
a. What is the complementary strand?
b. Deduce the mRNA in this coding region.
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