In vitro experiments are conducted at pH = 7.4 to simulate physiological conditions. A phosphate buffer system is often used. H₂PO → H₂PO¹²¯ +H+ pK₁ = 7.2 a. What must be the ratio of the concentrations of HPO to H₂POд ions? b. What mass of NaH2PO4 must be added to 500.0 mL of 0.10 M Na₂HPO4 (aq) in the preparation of the buffered solution?

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter13: Dimensional Analysis/units Conversion
Section: Chapter Questions
Problem 2.4P
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In vitro experiments are conducted at pH = 7.4 to simulate physiological
conditions. A phosphate buffer system is often used.
H₂PO → H₂PO¹²¯ +H+ pK₁ = 7.2
a. What must be the ratio of the concentrations of HPO to H₂POд ions?
b. What mass of NaH2PO4 must be added to 500.0 mL of 0.10 M Na₂HPO4
(aq) in the preparation of the buffered solution?
Transcribed Image Text:In vitro experiments are conducted at pH = 7.4 to simulate physiological conditions. A phosphate buffer system is often used. H₂PO → H₂PO¹²¯ +H+ pK₁ = 7.2 a. What must be the ratio of the concentrations of HPO to H₂POд ions? b. What mass of NaH2PO4 must be added to 500.0 mL of 0.10 M Na₂HPO4 (aq) in the preparation of the buffered solution?
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