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- 80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pI. The solution is then titrated with 0.2M HCL to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCL needed to reach relevant pKa value and equivalence point(s)z Relevant pKa values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.10 tab of acetaminophen 500 mg molar absorptivity= 14520 @ 243nm. The total weight of the 10tab is 5278 mg. I take a sample equivalent to 100mg of tablet and I diluted it in 100mL, I took an aliquot of 10mL and I diluted it in 250mL, of these I took 25mL and I diluted them in 250mL. Calculate the weight per tablet. Absorbance = 0.944The isoelectric point of eIF4a is 5.02. Students are given a sample of cell lysate containing eIF4A at pH 7.4. They are also provided with two buffers of pH 7.4 to use for ion exchange chromatography. One buffer has a very high salt concentration (1 M), the other has a low salt concentration (0.1 M). Mariela decides to use an anion exchange column, while Ashok chooses a cation exchange column. Who made the better decision? Provide a detailed explanation of why one student will end up with a purer sample of eIF4A. Which buffer will you use while washing away impurities (high or low salt), and which would you use to remove eIF4A from the column? Explain your choice.
- A 5% dextrose in 1/2 normal saline (D5 1/2 NS) solution is commonly administered to patients needing post-operative IV fluids. How could you prepare 500 mL of this solution? Dextrose is 5% (m/v) and the NaCl is 0.45% (m/v).Calculate the pH of a buffer that contains 0.15 M MOPS anionic form and 0.25M MOPS zwitterion. The pKa is 7.2Calculate the concentration of 0.2ml amino acid when path length is 1cm and has an absorbance value of 0.50, if a 0.5ml solution of same amino acid has a concentration of 0.72mg/ml with an absorbance of 0.9.
- Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength.Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength. Answer: You did not give the correct unit. The correct answer is: 333 mL XWhat volume of 0.1M NaOH (reagent) needs to be added to increase your 7.5 pH (buffer) by 0.5 pH units. The goal is to further purify MOPs (see image). Protonated form = 6.6 x 10.3^-3 mol/L Deprotonated form = 1.32 x 10^-2 mol/L Show calculations.
- Assuming the molar extinction coefficient for para-nitrophenyl phosphate (PNP) is 55,290 mol "dm cm, calculate the concentration of the molecule that produced an absorbance of 0.770 OD units in a 1.5 cm cuvette. Show all working -1 3 -1Guanosine (C10H13N5O5) in solution has a maximum absorbance at a wavelength of 275 nm. The molar extinction co-efficient at this wavelength is 84M−1cm−1and the path length is 24.7 cm. Through the use of a spectrophotometer, it is found that the that A275= 1.48. What is the concentration of the guanosine solution in grams/litre? Molecular weights (g/mol): C-12, H-1, N-14, O-16 Select one: A. 0.201869 g/L B. 0.435188 g/L C. 0.059919 g/L D. 0.294046 g/L E. 0.000713 g/LDescribe the preparation of 2.00 L of 0.100 M glycine buffer, pH 9.0, from glycine and 1.00 M NAOH. What mass of glycine is required, and what volume of 1.00 NaOH is required? The appropriate pK, of glycine is 9.6.