Developmental Biology
Developmental Biology
11th Edition
ISBN: 9781605354705
Author: Scott F. Gilbert, Michael J. F. Barresi
Publisher: Sinauer Associates is an imprint of Oxford University Press
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Chapter 12, Problem 2DQ
Summary Introduction

To review:

The reason for the rare presence of two different sets of DNA in the cells of a mammal assumed as perpetrator, in the crime scene of an episode of CSI: Crime Scene Investigation which is based on real instances.

Introduction:

The presence of cells with different genotypes in a single organism is known as genetic chimerism or chimaera. Animal chimeras are a result of merginging of multiple fertilized eggs. Chimeric condition arises from at least four parental cells i.e. two fertilized eggs or early embryos which are fused together. This situation leads to the population of cells which keeps their identity intact, thus resulting organism bears a mixture of tissues.

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Task 1: Compare the characteristics of DNA to RNA. Think before you drop - This is an all or nothing type of question. Double check that you are happy with where you placed all of the options before you submit the knowledge check. DNA RNA No Answers Chosen No Answers Chosen DNA & RNA No Answers Chosen Possible answers Phosphate group | Cytosine Adenine Basic unit is the nucleotide Single strand Double helix E Phosphate-sugar backbone | Uracil | Guanine | Thymine :::: ::::
WORKSHEET TASK 3: 1.     Below is a theoretical section of DNA. Design two primers that are 10 base pairs (bp) long that will amplify this section of DNA in a PCR reaction (‘N’ refers to non-specific ‘nucleotide’).     3’–A C G T G A A C T G C C T NNN......NNN C C G T G T A T C T C T T–5’   5’–T G C A C T T G A C G G A NNN......NNN G G C A C A T A G A G A A–3’
Question People who carry a theoretical genetic disorder (called B-disease) can be identified from a 2kb DNA sequence. People who carry this genetic disorder have a single nucleotide polymorphism that results in a change of GTATTC to GGATTC, a site that only occurs once at nucleotide number 750 in this DNA sequence. Answer the following questions based on the information provided. (a) How can you develop a simple molecular test to identify the genetic disorder? (b) If you have carried out the molecular test (based on the information above) on a 100 individual and found that 24 were healthy (BB) and 26 were carriers (bb); (1) What is the ratio of heterozygous? (2) Show how can you identify the three types from the agarose gel.

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