You have a cell count of 6.7 x 10° cells / ml in a total volume of 4.5ml. You have resuspended your cells in 5ml sterile PBS. You need to seed a six-well plate with the following numbers: a. 2 wells at 1 x 10" cells / well (2ml / well) b. 2 wells at 4 x 10 cells /well (2ml / well) c. 2 wells at 2 x 10° cells / well (2ml / well) Determine the volume of the original culture you will need to seed each concentration.

You have a cell count of 6.7 x 10° cells / ml in a total volume of 4.5ml. You have resuspended your cells in 5ml sterile PBS. You need to seed a six-well plate with the following numbers: a. 2 wells at 1 x 10" cells / well (2ml / well) b. 2 wells at 4 x 10 cells /well (2ml / well) c. 2 wells at 2 x 10° cells / well (2ml / well) Determine the volume of the original culture you will need to seed each concentration.

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter30: Assisting With Minor Surgery

Section: Chapter Questions

Problem 2CR

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B) A total of 2.5 x 105 cells were harvested from the patient. You need the cells to fill a scaffold with dimensions of 20mm x 20mm x 4mm at a final density of 2 x 104 cells/mm³. After culturing the harvested cells, you found that they have a doubling time of 36 hours. Determine the total number of cells (N) needed to fill the scaffold. Show your work.
You seed another culture of different end cell line, Walker12 cells, at 8 am on Friday with 10^4 cells. By Thursday at noon (12:00 pm) they reach confluence. Assume the sae confluency as stated above for a T150 flask (culture area of 150cm^2 adn when confluent contains 2 x 10^7 cells). What is the generatoin time (doubling time)? A. 120 hours B. 80 hours C. 38 hours D. 8 hours E. 11.4 hours F. other ________
B) A total of 2.5 x 105 cells were harvested from the patient. You need the cells to fill a scaffold with dimensions of 20mm x 20mm x 4mm at a final density of 2 x 104 cells/mm³. After culturing the harvested cells, you found that they have a doubling time of 36 hours. Given the growth rate is proportional to the number of cells, determine the time it will take to achieve the desired cell count. Show your work.
Imagine you repeated the counting of N. crassa conidial cells and found an average number of cells of 500. Before adding the cells to the hemocytometer, you mixed 100ul of conidial suspension with 100ul of water. You then added 10ul of this mix to the chamber. What is your density in cells/ml? O 10,000,000 cells/ml 2,500,000 cells/ml O 5,000,000 cells/ml O Impossible to determine
3) If Hybridoma cells are cultured supported in an agarose matrix in which nutrients and oxygen are supplied through thin equi-distanced parallel tubes that pass longitudinally along the agarose tissue bundle, name two appropriate boundary conditions to solve for maximum distance apart. Be sure to define what all the symbols and include a drawing.
Experiment: Page 6 de can exist stably in either a haploid or a diploid state. A haploid S. cerevisiae mes. When certain haploids come into contact, they fuse their cell walls and membranes, followed by the fusion of their nuclear membranes. The single nucleus now has 32 chromosomes, 16 from each parent strain, and is thus a diploid. Haploid yeast strains divide mitotically to give rise to haploid progeny, and diploid strains divide mitotically to give rise to diploid progeny. Certain haploids can fuse to form diploids. Haploid S. cerevisiae exists in two "mating types," called a and a Mating occurs only between a and a cells; no mating occurs between cells of identical mating type. We have a collection of eight a haploid mutant strains and eight & haploid mutant strains of yeast unable to synthesize tryptophan (trp). These will be combined (mated) in all possible combinations to yield diploid strains. If the diploids can grow on minimal medium, complementation occurred between the…
You are given a cell stock A. You make a 1:100 dilution and load 10µL diluted sample to a hemocytometer. After counting all 5 "one millimeter squares" you get an average of 60 cells. Then you decide to do a 1:1000 dilution to verify your counting. How many cells do you expect to get in average with 1:1000 dilution? Select one: a. 6 b. 12 с. 30 O d. 20 To do a 1:100 serial dilution. This is what you will do: Select one: a. You take 1uL of original cell stock and add into 99 uL of 1×PBS to make a 1:100 dilution b. You take 90uL of cell stock and add that into a tube containing 10uL of 1x PBS. c. You take 99uL of cell stock and add that into a tube containing 10uL of 1×PBS. d. You take 10uL of original cell stock and add into 90 uL of 1x PBS to make a 1:10 dilution. You thoroughly mix the 1:10 dilution tube. Then you take 10uL out from 1:10 tube and add it into a new tube containing 90uL of 1×PBS.
Imagine you have been given a liquid culture of yeast with a starting concentration of 3.67 x 10' cells/ml and are asked to carry out the sample dilution process shown in the figure below. 100μl 100μl 100μl 100μl 100μl 0.9ml 0.9ml 0.9ml H2O H₂O 6.9ml 0.9ml H₂O H₂O H₂O Original 10-1 102 10-3 104 Culture 105 100μl 100μl 100μl Plate A Plate B Plate C a. How many colonies should have been present on Plate A in this example? - Answers must be whole numbers as partial colonies are not expected. b. Imagine you carried out the same dilution scheme shown in the figure above, but now, you do not know the concentration of the original culture. If you counted 163 colonies on Plate B, what is the concentration of cells/ml in the original culture?
The following image shows part of counting chamber you used to estimate cell viability for yeast cells. If the dilution factor you used was 1:200, and the total sample volume was 5 ml, then calculate the cell viability, the cell density and the total cell.
Describe how you would prepare a dilution series of a 1 x 107 CFU/mL culture to the 10-8 dilution using only 4.5 mL diluents in tubes. What would be the theoretical cell count if 0.1 mL of the 2nd and 4th dilutions were each plated?
Draw cells in 4 large squares of the hemocytometer showing: 48 total viable cells (hollow dots) 9 total nonviable cells (shaded dots) 2 viable cells that are not counted 3 nonviable cells that are not counted
continuous disc stack centrifuge is operated at 5000 rpm for separation of tukers' yeast. At a feed rate of 60 min21, thirty of the cells are recovered. For operation at constant centrifuge speed, solids recovery is inversely proportional to the flow rate. (a) What flow rate is required to achieve 90% cell recovery if the centrifuge speed is maintained at 5000rpm thi What operating speed is required to achieve 90% recovery at a feed rate of 60 min211).