A lab trainer instructed a new lab recruit to freeze down cells from a tissue culture flask to save for future use. After verifying that the flask of adherent cells was confluent, and contained enough cells to freeze down, the lab trainer left the recruit to go through the freezedown protocol. A few days later, the trainer thawed out the cryovials of cells that the recruit put away, and discovered that there were very few viable cells. Assuming that the lab trainer did everything correctly, answer the following: What are two possible mistakes made that explains why there are so few cells? Explain why those mistakes had the effect they did.
A lab trainer instructed a new lab recruit to freeze down cells from a tissue culture flask to save for future use. After verifying that the flask of adherent cells was confluent, and contained enough cells to freeze down, the lab trainer left the recruit to go through the freezedown protocol. A few days later, the trainer thawed out the cryovials of cells that the recruit put away, and discovered that there were very few viable cells. Assuming that the lab trainer did everything correctly, answer the following: What are two possible mistakes made that explains why there are so few cells? Explain why those mistakes had the effect they did.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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A lab trainer instructed a new lab recruit to freeze down cells from a tissue culture flask to save for future use. After verifying that the flask of adherent cells was confluent, and contained enough cells to freeze down, the lab trainer left the recruit to go through the freezedown protocol. A few days later, the trainer thawed out the cryovials of cells that the recruit put away, and discovered that there were very few viable cells. Assuming that the lab trainer did everything correctly, answer the following:
- What are two possible mistakes made that explains why there are so few cells?
- Explain why those mistakes had the effect they did.
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