You analyze a protein of 100 kDa using SDS-PAGE in the absence and presence of �-mercaptoethanol (BME) and observe the following band pattern in the gels:   Which of the following statements about the protein is correct? (the image is attached) a. The protein consists of three polypeptide chains, two of which are connected via S-S bridges. b. The protein consists of two different polypeptide chains connected via S-S bridges. c. The protein has two different folding conformations. d. The protein consists of two different polypeptide chains linked to each other via non-covalent interactions.

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter5: Unit, Percentage, Milliequivalent, Ratio, And Household Measures
Section: Chapter Questions
Problem 1.7P
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You analyze a protein of 100 kDa using SDS-PAGE in the absence and presence of �-mercaptoethanol (BME) and observe the following band pattern in the gels:

 

Which of the following statements about the protein is correct?

(the image is attached)

a. The protein consists of three polypeptide chains, two of which are connected via S-S bridges.

b. The protein consists of two different polypeptide chains connected via S-S bridges.

c. The protein has two different folding conformations.

d. The protein consists of two different polypeptide chains linked to each other via non-covalent interactions.

 

 

 
kDa
70
45
25
no BME
protein
markers sample
10
||
|||
kDa
70
45
25
with BME
protein
markers sample
10
|||
||
Transcribed Image Text:kDa 70 45 25 no BME protein markers sample 10 || ||| kDa 70 45 25 with BME protein markers sample 10 ||| ||
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