Which of the following do we NOT look at when determining results of a TSI a. Color of the butt b. Color of the slant Liquification d. Gas bubbles/breaks in the agar O2 released from Citrate utilization causes the media to become more a. Acidic b. Alkaline C. Neutral . Liquid of beau si taet viviliensa entation can result in two different end products, Aleghols, acids Ketones, bases SP
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- Catalase: 1. Explain the incubation conditions 2. Explain the reagents being added 3. Explain the observations in Catalase 4. Explain the interpretations in CatalaseUrease: 1. Explain the incubation conditions 2. Explain the reagents being added 3. Explain the observations in Urease 4. Explain the interpretations in UreaseA. Observation of growth characteristics on selective and differential media Mannitol salt Agar Bacteria Growth (+/-) Describe growth characteristics Interpretation S. aureus E. coli P. aeruginosa M. luteus
- do methodologies preparation of the following 6 solutions (Broth LB, LB Agar, Potato-Dextrose Agar, LB-Dextrose Broth, Saline solution 0.85%, Glycerol 80%). Include photos or schematics.B. Microbial Processes Study the diagrams and answer the given questions. Pepartery For items no. 1 to 6, refer to the diagram at Glucose the left corner. 1. What process is shown in the diagram? 2. In what part of a bacterium does the process occur? 3. What are the raw materials of the process? Dnydryacetore phoghee (DHAP Enerr eenserving stage 2NAD NADH 4. What stage involves the consumption of two ATP molecules? 2000-0 synopen 5. What stage involves the production of four ATP molecules? Phoy PEP ZADP Pyuv aeMatch inhibitors, selective ingredients, pH indicators and other components to correct medium phenol red pH indicator [ Choose ] [Choose] mannitol Мас Sabouraud Agar 7.5 g NaCI/100 ml inhibitor Blood agar MSA low pH, high dextrose concentration [ Choose ] inhibitors neutral red pH indicator [ Choose ] crystal violet and bile salt inhibitors [ Choose] no inhibitors [Choose ] lactose as only sugar in medium [ Choose ]
- The following is a Mannitol Salt Agar plate. What does the yellow color on the right side of this plate indicate? TAW ASM's MicrobeLibrary Ⓒ Reynolds tolerance of the salt mannitol metabolism tolerance of the dyes and chemicals in the agar lactose metabolismMatch the description to the correct medium This medium is enrichment for fastidious [Choose ] bacterial pathogens and differential based on hemolysis [Choose ] This medium is selective based on Tryptic Soy Agar, TSA salt/sodium chloride tolerance at 7.5 % Sabouraud Dextrose Agar, "Sab" agar weight/volume and differential based on mannitol fermentation Mannitol Salt Agar, "MSA" MacConkey Agar, "Mac" agar This medium is selective based on the ability to grow in bile salts and crystal violet and is EMB Eosin Methylene Blue Agar differetial based on lactose fermentation Blood Agar, "BA" this medium is all purpose and can grow a [Choose ] wide range of bacteria and fungiGive the use and advantages of the following cultures for the examination ofcolony characteristics: a. Agar plateb. Agar slantc. Broth cultures
- B. Microbial Processes Study the diagrams and answer the given questions. Preparatory stage 000000 For items no. 1 to 6, refer to the diagram at Gucone the left corner. of 0000 Fudese dghophate Dihydoyactone phosphaie (DHAP) Energ conserving stage 2 NAD 4. What stage involves the consumption of two ATP molecules? 2 NAD 000-O 134phoaphogyceric acid 5. What stage involves the production of four ATP molecules? sphosphogyoeric acid 6. What are the products of the process? 2 phosphogyoeric acid Phosphoenoipyravie acid (PEP) Pynae acidSuspend 28.0 grams in the 1000 mL distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (1210C) for 15 minutes. Cool to 45-500C. If desired the medium can be enriched with 5-10% blood or other biological fluids. Mix well and pour into sterile Petri dishes. 1. Determine the amount of dehydrated medium needed to prepare 50 nutrient agar plates. Include amount for 2 additional plates as excess to compensate for compounding losses.1. Give the importance and purpose of the Cetrimide Test. 2. Discuss briefly how each of the different oxidase method is being performed - Dry Filter Paper Method, Wet Filter Paper Method and Swab Method. 3. In the Oxidative-Fermentative Test, differentiate non-saccharolytic from oxidative from fermentative results.