Urease: 1. Explain the incubation conditions 2. Explain the reagents being added 3. Explain the observations in Urease 4. Explain the interpretations in Urease
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Urease:
1. Explain the incubation conditions
2. Explain the reagents being added
3. Explain the observations in Urease
4. Explain the interpretations in Urease
Step by step
Solved in 3 steps
- Catalase: 1. Explain the incubation conditions 2. Explain the reagents being added 3. Explain the observations in Catalase 4. Explain the interpretations in CatalaseA. Explain why it is essential to digest all wild-type pQE. 1.-CRYGD. B. How would you make 50 mL o 1% weight per volume (w/v) agarose solution?H. Consider the answers you provided in the questions above in order to answer the following question. You treat a suspension of Stabhylococcus aureus with lysozyme enzyme dissOved 25 mM Tris buffer (pH 7.4). After a 30-minute incubation, the cells lyse completely. You treat a suspension of Pseudomonas aeruginosa with the same lysozyme/Tris solution and get no lysis after a one-hour incubation. Why? Provide a detailed answer based on your understanding of
- Explain Emulsions. What variations are there? Give an illustration of each category. Create two emulsification programmes.CATALASE b.how will you inoculate? c.How will you incubate? d. what are Post- Incubation procedures? e. How will you read the positive and negative results?INDOLE b.how will you inoculate? c.How will you incubate? d. what are Post- Incubation procedures? e. How will you read the positive and negative results?
- C. d. Lactic Acid Red dye #4 Which of the following do we NOT look at when determining results of a TSI tes a. Color of the butt b. Color of the slant Liquification d. Gas bubbles/breaks in the agar CO2 released from Citrate utilization causes the media to become more a. Acidic b. Alkaline c. Neutral d. Liquid of beau mentation can result in two different end products, Aleohols, acids Ketones, bases Tryptophan, alcohol sodium caf Viviliensa r ar2. What are the substances added in the culture media utilized by the organism producing hydrogen sulfide? Give 2 other media used for the production of hydrogen sulfide. 3. Explain the mechanism taking place in the hydrolysis of urea which leads to the formation of bluish red color. 4. Discuss why human blood plasma will not always yield reliable results.6. Serum blood of a patient with dislipoproteinemia type I has milky appearance even in fasting. If serum stays at low temperature (4) for several hours, the fatty layer appears on its surface. What are the possible causes of these symptoms? Answer the questions and do the following tasks: a) what compounds of serum blood must be tested in the patient in a biochemical lab? b) what is the possible diagnosis of the discase? c) draw the reaction which does not occur properly in the patient's blood; d) explain how the products of the previous reaction are used in adiposc tissue and heart in a healthy person I hour after meal.
- GELATIN b.how will you inoculate? c.How will you incubate? d. what are Post- Incubation procedures? e. How will you read the positive and negative results?Enzymes MATCHING TYPE a.Erepsin b.Sutilains c.Chymotrypsin d.Urokinase e.Fibrinolysin f.Rennin g.Papain h.Alcalain i.Bromelains j. Pepsin 1.Additive to remove protein stains 2. Relieve symptoms of episiotomy 3. Isolated from human urine or obtained from human kidney cells by tissue culture techniques. 4. Treatment of blood clots within the cardiovascular system, exclusive of thrombi of the coronary and cerebral ateries. 5. Obtained from the stomach of Sus scrofa, Fam. Suidae and has a proenzyme: pepsinogen which is activated by HCl. 6. From Bacillus subtilis, used as wound debridement. 7. Coagulating enzyme that is present in the mucous membrane of the stomach of animals. Important in cheese making. 8. Crystallized from an extract of the pancreatic gland of Ox Bos Taurus used as an ophthalmic solution. 9. Mixture of protein-digesting & milk clotting enzymes from the juice of Ananas comosus used as meat tenderizer. 10. Found in the intestinal juice, Converts proteoses and…Answer the following questions in full sentences and paragraphs: 1. Diagram (draw) the Gram positive cell wall. Be sure to label specific components of the structure. What is the medical significance of LTAs? 2. Diagram (draw) the Gram negative cell wall. Be sure to label specific components of the structure. What is the medical significance of LPS? What is the medical significance of the O-linked side chain?