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Which medium is well-indicated to amplify a small population of bacteria mixed with much larger bacterial colonies?
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- Why should bacterial culture plates be incubated inverted (bottom up)?Mannitol salt agar is often used to distinguish between different species of Staphylococcus, a gram positive bacterium that is well adapted to living on dry, salty skin. Disease-causing strains of Staphylococcus ferment mannitol; non-pathogenic strains cannot use mannitol. Is the medium Defined or Complex?You've been tasked with observing the motility of an UNKNOWN Bacteria isolated from a mixed culture. Which technique(s) is/are the BEST to utilize? How will the procedure be carried out? Explain.
- Does agar concentration affect the growth and morphology of bacterial colonies? What causes dense growth and what causes minimal growth?Starting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium with a 1-hour lag phase and a 30-minute generation time, how many cells will there be in a liter of medium at the end of 2 hours? At the end of 7 hours? Show your solution.In the experiment by Bernard Davis, bacterial F+cells and F- cells were growing while separated by a filter. Filter pores allowed the passage of the liquid medium but not the bacteria cells. As a result: 1) prototype colonies grew well on minimal medium 2) F+ cells were converted to F- cells despite the physical separation 3)F- cells were converted F+ cells despite the physical separation 4)F+ cells were not converted to F- cells because of the physical separation 5) F- cells were not converted to F+ cells because of the physical separation 6)there was no growth of prototypes on minimal medium
- Why are complex media preferable to chemically defined media for routinecultivation of microorganisms?What general type of growth medium would you use to: (a) grow one type of bacteria but inhibit the growth of another type? (b) discriminate between different types of bacteria?Heat-labile material to be introduced to culture medium was filtered using membrane filters with unknown pore size. Effect: Rationale: