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- 1. Describe the three different type of hemolysis that are observed on blood agar.2. What is a selective medium?3. What is a differential medium?4. Which media can be used to isolate E. coli samples from contaminated lettuce?5. Which two media would be used to identify a sample taken from a patient with suspected gonorrhea?6. Would you be able to grow a sample obtained from a patient's wound (suspected to be infected with MRSA) on EMB? Explain.7. What is the color or TSI for Salmonella?8. What is a fastidious organism?1. Give 2 systems used at present for the automatic identification of bacteria. 2. Why aseptic technique important in urine collection for culture? 3. What are the specimen consideration you have to remember in preparing blood culture? 4. Being a medical technology student, how can you contribute to avoid water pollution and degradation of natural resources?1. What is the critical threshold between bacterial infection and simple bacterial contamination? 2. Why is aseptic technique important in urine collection? 3. Give at least 5 bacteria that can cause Urinary Tract Infection.
- 1. Differentiate between Gram positive and Gram negative cells in smears. 2. Discuss how to properly prepare a bacterial smear.1.)What is the purpose of a counterstain? 2. What does a mordant do in the Gram stain procedure? Which reagent in the Gram stain is the mordant? 3. True or False? The oil objective should make contact with the oil on the slide. 4. Why is it necessary to let bacterial smears completely air dry before heat fixing? 5.Why should controls be included wherever possible for any staining technique? 6. Why is it necessary to heat the slides while staining for endospores?1. Illustrate in a diagram the identification scheme from gram-positive cocci.2. Discuss the principle involved in the biochemical tests in identifying species of staphylococci.3. Illustrate in a diagram the identification scheme for Streptococci.4. Discuss the principle involved in the biochemical tests in identifying species of Streptococci.
- 20. Starting with the MacConkey agar and IMVIC tests. Describe how a bacterium can be biochemically identified as Escherichia coli.I. Match the bacterium being described in column X with its best classification found in column Y. Choose the best answer. Column X1. Staphylococcus aureus can be grown on mannitol salt agar that contains 7.5% NaCl. The bacterium is________. 2. Lactobacillus acidophilus grows best at pH 4.5. It is considered a(n) ________. Column Y A. acidophile B. alkalophile C. barophile 3. A bacterium that thrives in the Great Salt Lake but not in fresh water is probably a ________.4. Bacteria isolated from the bottom of the ocean need high atmospheric pressures to survive. They are________.5. A bacterium that thrives in a soda lake where the average pH is 10.5 can be classified as a(n) ________. D. halotolerant E. halophileWhat are two possible reasons for choosing a bacteriostatic treatment over a bactericidal one? Name the factors that can compromise the effectiveness of a disinfecting agent
- A 1.5-year-old child developed vomiting, diarrhea, and fever. Stool sample were inoculated into the Endo media. After 18 hours on the surface of the medium grew medium-sized, round, slightly convex red colonies with a metallic luster. The doctor suspected Escherichia coli. 1. Name the composition of the Endo agar media. 2. Describe the properties of bacterial colonies on the Endo media. 3. What purpose differential diagnostic Endo media used for?15. What are the IMVIC series of tests? WHY / HOW are they used? 16. What is the catalase test? How is it used for colonies that are gram positive?13. What is a differential agar? Why can it be of importance? 14. Describe how the following agars work: MacConkey, Blood, Phenylethyl alcohol, Mannitol salt. 15. What are the IMVIC series of tests? WHY / HOW are they used? 16. What is the catalase test? How is it used for colonies that are gram positive?