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- . A certain medium has the following composition: Glucose 15 g Yeast extract 5 g Peptone 5 g KH2PO4 2 g Distilled water 1,000 ml Tell what chemical category this medium belongs to, and explain why this is true. How could you convert Staphylococcus medium (table 3.6A) into a nonsynthetic medium? a. Name four categories that blood agar fits. Alpha hemolysis Beta hemolysis Gamma Alpha prime Name four differential reactions that TSIA shows. Observe figure 3.15. Suggest what causes the difference in growth pattern between nonmotile and motile bacteria. Explain what a medium that is both selective and differential does, using figure 3.18. a. What kind of medium might you make to selectively grow a bacterium that lives in the ocean? MSA Mannitol salt agar One that lives in the human stomach? Why are intestinal bacteria able to grow…Oxidase Test:In the document you submit, please describe the results for this test… (like yellow equals what?) 1) Give me the expected results for Escherichia coli and Pseudomonas aeruginosaa. What oxygen requirements do your think this bacterium will have (obligate aerobe, obligate anaerobe, facultative anaerobe, aerotolerant anaerobe, or microaerophile), and why? b. You run a thioglycollate test of your isolated bacterium. Based on your response to Part A, which thioglycollate tube best represents the result you would expect for your bacterium? Explain your response. If the result you would expect is not represented, explain what the tube should look like. c. What would you expect to be the result of a catalase test of your bacterium? d. What techniques or specialized equipment, if any, would you need to grow cultures of this bacterium?
- Background: The following results were obtained from a broth dilution test for microbial susceptibility. What is the minimum bactericidal concentration of this antibiotic and explain what you based your answer on?a. Using table as a reference, find and explain the differencesbetween the results for Staphylococcus and Pseudomonas. Are anyof these drugs broad spectrum?b. Explain the difference in the MIC of E. coli for penicillin versusampicillin.B. COLOR REACTIONS. Give the ideal observation for the positive results of the following samples to complete the table below. Write NO RXN if the sample gives negative result for the given tests BIURET XANTHOPROTEIC NINHYDRIN SULFUR SAMPLES TEST TEST TEST TEST Formation of violet- Cysteine NO RXN colored product Formation of red- Tryptophan NO RXN colored solution Formation of Formation of violet- Keratin black colored product precipitate B - Alanine NO RXN NO RXN
- Identify which antibiotic was used per set-up (see figure). Describe the result in the graphs provided to help you explain your answer. Listed below are some essential information.Antibiotic A: 0.5 kDa protein, targets peptidoglycanAntibiotic B: 20 kDa protein, targets peptidoglycanAntibiotic C: Cationic antimicrobial peptideAntibiotic D: Targets lipopolysaccharideStaphylococcus aureus: gram-positive bacteriumVibrio cholera: gram-negative bacteriumMethanosarcina: an archaean bacteriumCationic antimicrobial peptides (CAMPs): these positively charged antibiotics are attracted to the negatively charged cell wall and membrane. They are hydrophobic, and they insert into the membranes to create pores.Answer the following questions in full sentences and paragraphs: 1. Diagram (draw) the Gram positive cell wall. Be sure to label specific components of the structure. What is the medical significance of LTAs? 2. Diagram (draw) the Gram negative cell wall. Be sure to label specific components of the structure. What is the medical significance of LPS? What is the medical significance of the O-linked side chain?Given this, if you used 6g of vitamin Z powder to make 20 ml of solution, what is the % concentration of this solution? (I gave the image since I don't know if that info is needed to solve this question.)It also gives a follow-up, if you can help here too: You work in a lab as a summer student. One of your tasks is to make sure that there is enough cell culture medium containing antibiotics to grow bacteria. One day you realize that there is only 5 ml of 10% Antibiotic stock solution in the freezer. You decide to use it all to prepare the working culture medium with 0.01% antibiotic. In the lab there is plenty of growth medium without antibiotics. (Note: dilution in medium is like dilution in water). You remember the equation to make dilutions of stock solutions. You usually use this formula to calculate the required volume of a stock solution, but you realize it can apply here as well, even though the unknown is the final volume. So, you make that dilution. Given that each bacterial…
- Please give a brief and clear answer with your own words. Question: Define methods ( MEM Extraction, Agor Diffusion, Direct Contract) to test biocompatibility (cytotoxicity). Compare/contrast their advantages and disadvantages.and the minimum exposure time 1. Determine the minimum inhibitory concentration given the following data: Concentration of ethanol Number of colonies of E. coli on Nutrient Agar plates Exposure time (in minutes) 10 5 15 Distilled water +++ +++ +++ 40% +++ ++ + 70% 95% Minimum inhibitory concentration: Minimum exposure time:Determine the minimum inhibitory concentration and the minimum exposure time given the following data: Number of colonies of E. coli on Nutrient Agar plates Exposure time (in minutes) Concentration of ethanol 10 15 Distilled water +++ +++ +++ 40% +++ ++ 70% 95% Minimum inhibitory concentration (MIC): Minimum exposure time (MET):