Total protein: Do you think that refractive index could be used as a regular method for serum protein determinations? If yes, what limitations does it have. If not, why not? What are the major interferences in the biuret method? Refractometry method?
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Total protein:
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Do you think that refractive index could be used as a regular method for serum protein determinations? If yes, what limitations does it have. If not, why not?
-
What are the major interferences in the biuret method? Refractometry method?
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- What are the advantages of granulation ? during tablet formulation. DefineConsider the fluorescence spectra for bovine serum albumin (BSA) below. One solution contains phosphate buffered saline (PBS), while the other contains 1M HCL. Why does the fluorescence change the way it does? I.e., why do we observe a higher peak and a shift to the left under the presence of acid?Given that the normal range for serum protein is about 7 g/100ml and albumin is about 4 g/100ml, what clinical conclusions can you draw from the following samples A-C? If samples are not normal, what protein fraction is increased/decreased and with what disease is this protein pattern likely to be associated? Sample A: total protein = 14 g/100mlalbumin= 8 g/100 ml Sample B:total protein = 5.8 g/100ml albumin = 4 g/100 ml Sample C:total protein = 3.5 g/100mlalbumin = 2g/100 ml
- After blood collection, the red cells are separated from the serum to be used for the preparation of the stock solution. How is it done? For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. What is the appropriate diluent used for the preparation of the red cell suspension?6) 1 mL of supernatant is required for a procedure. The final colored solution proves to be too high to read accurately on the spectrophotometer. 100 μL of supernatant and 900 μL of distilled water are substituted for the original supernatant and the procedure, run as before. The reading from the standard curve is 46 mg/dL. What is the actual amount of substance in the patient serum?How is 10^4 derived? Please show all calculation steps. # of cells per mL = (Average)*DF*10^4
- K.What would happen if you reversed the electrodes on the Gel box? What would you get look like after imaging?A.How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x? B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?I need to prepare a standard calibration curve for gamma globulin. absorbances on Y and mg of standard protein per assay on X. used 0.1mg/ml gamma stock for tubes 2-6. (Water (ml), gamma (ml), Abosrbance)--> (.036, .004, .290) (.036, .008, .358) (.024, .016, .341) (.016, .024, .520) (.008, .032, .597) - What is the math and how do you get the standard curve?
- For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. What are the initial concentrations used for tubes 3, 5, and 6? Show pertinent solutions. What are the dilutions of the last positive tube and first negative tube respectively? Show computation.Consider the following prescription: Sol silver nitrate 0.5% 15mL Make solution isotonic for ophthalmic use. B. How many milligrams of potassium nitrate will need to be added to the prescription to make it isotonic? Report numerical value only rounded to a whole number. Need only handwritten solution only (not typed one).Table 5: Effect of Dietary Fiber on Bile Acid Adsorption Optical Density (OD) of Colored Filtrate (Reflects Amount of Bile Acid in Filtrate) 1- OD (Reflects Amount of Bile Acid Adsorbed by Food Particles) 000 Fiber- Fiber- Fiber- Fiber- Bile Acid Standard Standard Rich Rich Bile Acid Standard Standard Rich Rich With Buffer Meal Meal Meal Meal With Buffer Meal Meal Meal Meal Experimental Solution (No Trials With Bile Solution (No Fibers) Without Without With Bile Without With Bile Without With Bile Fibers) Bile Acid Bile Acid Bile Acid Bile Acid 1 1 0.55 0.4 0.00 2 1 0.61 0.4 3 1 0.52 0.32 Average 0.00 0.00 0.00 0.00 Equations (fx) Use these formulas for making your calculations. Round your answer to the nearest tenth. Averages Add the values from the three trials (or subjects); divide by three. Index reflecting the level of bile acid adsorption This index is equal to 1 - OD, where OD is optical density of the colorized supernatant of mixtures of the studied food. This index reflects…