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- GTP or ATP is produced during the conversion of isocitrate into ketoglutarate succinyl CoA into succinate fumarate into malate malate into oxaloacetate1. Consider the reaction: succinyl-CoA succinate H3C C H₂ a. What kind of reaction is being performed here? H³C C H₂ b. What enzyme performs this reaction? S-COA c. What cofactors, if any, are required for this reaction?8. The enzyme thiolase catalyzes one step in the ß-oxidation of saturated fats. One portion of the mechanism for this reaction is shown below. Describe the catalytic mechanism at work. (Note: "SCOA" is shorthand for the compound Acetyl-CoA) RCOCH₂COSCoA + HSCOA → H3C₂OSC0A + RCOSCOA HN NH CH₂ NH CH₂ CH₂ FS Grease NH3 H-SCO A NH CH2 CH₂ NH3 S Co A CH₂ NH 1 3 H-SCO A H3C SCO A NH3 a h-SCOA R-C S Co A CH₂ CH₂ C H₂ 0=0 HN NH + NH3 H₂C HN CH₂ NH CH₂ CH₂ 2 S Co A CH₂ NH NH
- 19. Which of the following statements is true for the shown reactions? i C-S-COA CH₂ H₂C-C-OH CH₂ coo W Coo CH₂ C=0 I Acetyl-CoA CH3 x 214 Coo- 1 CH ₂ I CHOH CH3 Y A. Deficiency of oxaloacetate stimulates the formation of X and y B. Insulin signaling stimulates the formation of X and Y C. Both A and B D. Neither A nor BCHOOSE THE CORRECT LETTER 1.What are the B-oxidation products of lauric acid (C12H2402), a major component of coconut oil?A.6 acetyl-CoA, 6 NADH, 6 FADH2B. 6 acetyl-CoA, 5 NADH, 5 FADH2C. 5 acetyl-CoA, 6 NADH, 6 FADH2D. 5 acetyl-CoA, 5 NADH, 5 FADH2 2.What is the mechanism of ATP synthesis in glycolysis?A. substrate level phosphorylationB. reductionC. oxidationD. oxidative phosphorylation 3.Which of the following enzymes need ATP as a substrate in the reaction?A.phosphoglycerate kinaseB. phosphofructokinaseC. pyruvate kinaseD.glyceraldehyde-3-phosphate dehydrogenase4. Identify: for nos. 1-5: Name of the missing metabolite in the pathway 6-10: Enzyme that catalyzed the reaction 11-15: Reaction involved 5 FAD 10 -FADH₂ trans-2-En oyl-CoA 15 H₂O 3-Hyroxya cyl-CoA NAD+ NADH+H+ beta-Ketoacyl-CoA COASH Acetyl-CoA Acyl-CoA (2 C Atoms Shorter) Malate Fumarate Glucose 6 Glucose-6-phosphate 11 Fructose-6-phosphate Phoshphoenolpyruvate Succinate 12 Acetyl ČOA 13 7 Citrate 3 Lactate 14 Isocitrate 8 a-ketoglutarate
- Consider the docosanoic acid, C21H43CO2H a. Label the a and B carbons b. Draw the acyl CoA derived from this fatty acid c. How many acetyl CoA molecules are formed by complete B-oxidation? d. How many cycles of B-oxidation are needed for complete oxidation? e. How many molecules of ATP are formed from the complete catabolism of this fatty acid?1. Biotin is an essential cofactor for carboxylases that transfer carbon dioxide. Choose one of the enzymes and describe the reaction and the role of biotin 2. Describe and discuss the metabolic roles of coenzyme A (CoA).9. The equation below depicts the first step of the citric acid cycle. H0 CoA-SH CH-C + 0=C-CO0 HO C-COO citrate s-COA ČH2-COO synthase ČH2 -CO0 Citrate Acetyl-CoA Oxaloacetate AG" = -32.2 kJ/mol a) Explain the chemical conversions that take place during this step. b) Why is this reaction energetically favorable? Explain.
- Part A Identify each of the following metabolic pathways. Drag the appropriate labels to their respective targets. Reset Help the conversion of fatty acids to 2-carbon units of acetyl CoA ketogenesis the conversion of an amino acid to an a-keto transamination acid the combination of 2-carbon units of acetyl COA to form fatty acids oxidative deamination the removal of an amino group as NH4+ from B oxidation glutamate to yield a-ketoglutarate lipogenesis the production of ketone bodiesCoenzyme A (CoA-SH) plays an important role in the Pyruvate Dehydrogenase Complex reaction. Which is correct? Coenzyme A serves as an electron acceptor in this reaction. The hydrolysis of a CoA thioester has a positive AG", the hydrolysis is endergonic and unfavorable. Coenzyme A is derived from thiamine, a vitamin. Coenzyme A contains -SH group and can serve as an acyl group carrier.B1. Complete the Kreb's Cycle Acetyl CoA Citric Acd Malate dehydrogenase NADH NAD Isocitrate NAD Isocitrate Fumarase dehydrogenase NADH Fumarate NAD" FAD COASH Succinate dehydrogenase Succinate Succinyl CoA Succinyl CoA synthetase B2. How many ATP molecules can be produced from 4 molecules of Acetyl CoA if all of them enter the Kreb's Cycle? Assume all the energy yielding products enters the electron transport chain.