what are the buffers used for biological and clinical measurements? state the properties of each buffer and the limitation to its applications.
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- Match the common laboratory scenarios to the correct buffer required using the guide below. Molecule pKa Value(s) Acetic acid 4.8 Carbonic acid 6.1, 10.2 Citric acid 3.1, 4.8, 5.4 HEPES 7.5 Phosphoric acid 2.1, 7.1, 12.3 TRIS 8.3 Mammalian cells require a pH of about 7.4 for cell and tissue culture experiments. Elution of remaining molecules from a cation exchange column requires washing with pH 10 buffer. Dissolution of lysozyme requires pH 3 buffer to maintain activity. Elution of remaining molecules from an anion exchange column requires washing with pH 2 buffer.…What is the minimum volume of dissolution media required to provide sink condition in dissolution test of a tablet dosage form (its weight: 1350 mg; dose strength: 1000 mg) regarding the following conditions? The molecular weight of an active drug substance is 500 Da; the dissolution media is water and the solubility of active drug substance in water is 10 mg/Ml (4. SLAYT) 500 ml 1000 ml 1350 ml 100 ml 135 mlShow workflow process of producing a Liquid Dosage Form through Extemporaneous CompoundingUse diagrams (boxes and arrows) to signify stepwise and significant procedures.
- Compute for the sodium concentration of the patient. Use the same formula in the computation of the concentration using absorbance and concentration of the standard. Given: Standard sample Concentration : 150mmol/L Absorbance: 0.112 Patient's sample Absorbance : 0.226 Concentration : ??A nurse wants to know the what volume of Water for Injection, BP to be added to a vial containing 500mg doxorubicin to produce an injection with a concentration of 2.5 mg/mL. The displacement volume of doxorubicin is 0.3 mL/10 mg.give the respective the TOTAL DILUTION FACTOR of respective serial dilutions. show complete solution.
- Make a list of the five most critical qualities of an effective extraction buffer. Please provide a brief description of each.Which is not a Limit test in the Sodium Chloride drug substance/raw material monograph? (Note: NaCl is used as a drug to counter hypotension (low blood pressure), and as a raw material in saline solution.)how to prepare a 100 mL of 4x sds buffer from a 1.0 L of 50x SDS stock solution. Show complete solution
- When a SBIA021 student measured the pH of 1.0M HCI, the student obtained a pH meter and failed to neither check the condition nor the manner of usage of the instrument. The pH meter reading timed out (Did not give a reading) Which of the following problem explanations is most correct? A)The sample in the glass beaker was being swirled vigourously while the electrode was submerged in it for a reading. B)The electrode got submerged much deeper into the solution than it should have. C)All of the above are correct problem explanations O D)The electrode had long direct contact with the environment and the glass membrane got dry.By applying Gravimetry, Titration (Chemical indicator) and Potentiometry lessons which technique should be used in following situations. Explain your answer. In your explanation, state what is the advantage of the technique you chose over other technique and /or the barrier/difficulty of using the technique. Your choice should not be dependent on cost. For chem students- Total Acid content per tablet of a newly developed drug that has yellow coating Target level is 1%.Calculate the CFU present in 150 mL water sample and b) dilution factor up to 5. Consider the following dilution scheme.