Q: The process that most accounts for magnification is a. a condenser c. illumination b. refraction of…
A: Magnification means the image produced is more than the size of the object.
Q: A microscope that has a total magnification of 1,500× when using the oil immersion objective has an…
A: The microscope is the major tool in the identification of a microorganism after its isolation. The…
Q: With the aid of a labelled diagram, outline the principle of the oil immersion objective
A: A microscope is an intrument which helps to enlarge microscopic images which are not visible to…
Q: While measuring the turbidity of a bacterial culture in class, Kevin recorded both the absorbance…
A: Turbidity is the measurement of the suspended particles in a fluid that makes it opaque. Quality…
Q: how foods are lyophilized and compare the advantages and disadvantages
A: Freeze drying also known as lyophilization is a method by which water is removed from frozen…
Q: Using pasteurization techniques as a model, compare the TDTs and explain the relationships between…
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: Explain the imporance of using a control tube with only broth when measuring turbidity in a…
A: Introduction :- There are two types of growth medium or media which are used to grow microorganisms…
Q: For post mortem stain to occur ______must happen. A. Coagulation B. Hemolysis C. refridgeration…
A: For post mortem stain to occur pH shift to alkaline must happen.
Q: To prevent any signs of contaminants in the broth it is sterilized by heating. Sterilization by…
A: A broth refers to the growing media for microorganisms. It consists of ingredients that are known to…
Q: In Gram staining technique, Decolorizing agent step should take Select one: O a. 60 seconds O b. 30…
A: Usually, Acetone is used as a decolorizer but some prefer ethanol and acetone in a 1:1 ratio.…
Q: Explain the principle involved in O.D determination of microbial populations using the…
A: A spectrophotometer is an instrument that can determine the concentration of a compound or solution…
Q: Describe the type of media you choose in the laboratory, use and prepare them ,, I want to describe…
A: 1. Glass slides on which the sample is placed. 2. Cover slip made up of plastic. 3. Dropper 4.…
Q: Sample diagram for the gram staining procedure and microscopic observation.
A: Danish Bacteriologist Hans Christian Gram in 1884 introduced differential staining techniques in…
Q: During studying a thick heavily stained specimen under the microscope, it is better to light…
A: Microscopy is the technique of visualisation of microscopic specimens by using a microscope after…
Q: Select ALL the correctly matched terms: a. Immersion oil/ Oil with a different refrative index than…
A: diffraction is a physical phenomenon in which the light waves bend when meet with an obstacle like a…
Q: Samples subjected to a test were given. Determine the result of the samples subjected to the given…
A: Samples subjected to a test were given. Determine the result of the samples subjected to the given…
Q: If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is…
A: Option b
Q: Which of these are used to measure optical activity?a) Polarimeterb) Planometerc) Psychrometerd)…
A: The optical activity is the process of the rotation of the polarization plane of a light beam when…
Q: What effect do you think using a greasy, oily, dirty slide will have on your judgement of the Gram…
A: Bacteria are the most important microorganisms to the food processor. Some bacteria are beneficial…
Q: This type of microscope is use to study the detail in samples. It produces a three-dimensional image…
A: A microscope is an optical instrument, which is used for observing small organisms and cells. It…
Q: Using a light microscope, samples can be viewed by which method (choose all that apply) O dissecting…
A: An optical microscope is another name for a light microscope.
Q: Three-dimensional images of specimens can be produced using z stacks obtained from confocal…
A: Microscopy is a technique that is used to visualize small-sized objects that are not used to the…
Q: Put the numbers in the space provided to indicate the order of Capsule Staining. If the statement is…
A: The primary function of capsule staining is to differentiate capsular material from bacterial cells.…
Q: If you have a substance that you wish to sterilize (without destroying), and that substance is…
A: Introduction Any procedure that eliminates, eradicates, or renders inactive all life…
Q: Is is best to observe slides of bacteria stained with the acid fast stain under high dry…
A: The acid-fast stain is used to stain the acid-fast organisms. Acid-fast microorganisms have a thick…
Q: Explain the importance of using a control tube with only broth when measuring turbidity in a…
A: A spectrophotometer is used to measure the turbidity of a microbial culture sample. It is also used…
Q: Membrane filters
A: Membrane filter are made up of cellulose.
Q: If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would…
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: The magnitude of the color differences between two objects is termed _
A: The magnitude of the color differences between two objects is termed _____________. Answer.…
Q: Compare and contrast chlorine, combined chlorine and UV light as disinfectants using a graphic…
A: Disinfection of different surfaces and objects can be done by a wide range of methods which can be…
Q: Define the following terms: a.Simple staining b.Complex medium c.Defined (synthetic) medium
A: Staining is used to visualise the bacteria i clinical specimen under a light microscope. Bacterias…
Q: The specimen for an electron microscope is always a. stained with dyes c. killed b. sliced into thin…
A: Electron microscope: It is a microscope which obtains a high-resolution image of non-biological as…
Q: Explain how a recrystalization will purify a solid. You MUST use relevant drawings in your answer.
A: Recrystallization: It is a technique performed in the laboratory to purify solids based on their…
Q: Your lab partner is observing a Gram-stained slide of their unknown organism with 1000X total…
A: In high magnification (1000X) the oil emulsion is used. It is used when we use 100X objective lens…
Q: An acid-staining method was performed on a bacterial smear using the dyes carboifuchsin and…
A: Acid Fast staining is used to detect the presence of acid-fast bacteria e.g. Mycobacterium…
Q: True or False: Ultrathin sections are used for light microscopy but are to thin to be stable under…
A: Microscopes are used to visualize objects that are not visible to the naked eye. In microbiology,…
Q: move the images of bacterial cells into the chart to illustrate the color of gram-positive and gram…
A: answer given below
Q: After performing the gram stain you observe your result as gram negative. To help confirm this…
A: Gram staining is a technique to identify bacterial stain gram-positive or gram-negative. It can be…
Q: what effect would failing to drain the smear in-between staining have on your judgment of the gram…
A: Gram staining is a technique used to differentiate between two groups of bacteria namely…
Q: Explain why dichloromethane is a good solvent to remove the fat from the milk? Can you use organic…
A: Dichloromethane is a good option for milk fat removal. The reason is: Dichloromethane is a polar and…
Q: You inoculate a culture into a test tube containing broth and take it out of the
A: Microbiological culture is a method of multiplying microorganisms in lab conditions on growth media.…
Q: Gram straining procedure. Indicate the following information A. Shape........ B. Stained color…
A: On the basis of gram staining, we can differentiate between two types of bacteria, one with thick…
Q: What are the expected result after gram staining? a. Crystal Violet = b. Gram Iodine = c.…
A: Gram staining is a type of staining technique used to differentiate bacteria based on their cell…
Q: household bleach reduces microbes because it is a member of what group of chemicals? a. halogen b.…
A: Small organisms that are not visible to naked eyes are called microbes or microorganisms. It…
Q: The raw product of an electron microscope is a color image of the object. True False
A: Introduction An electron microscope is a types of microscope where a beam of electron is used as…
Q: Formation and Composition of Film from Milk a. Place 10 mL of fresh milk in an evaporating dish.…
A: Milk It is a nutritious white liquid secreted by the mammary glands of mammals. It consists of…
Q: Which of the following terms describe how fast light travels through th medium? O All are correct…
A: Ans. Refractive index The bending of a ray of light as it passes through one medium and through…
Q: Explain how bacterial cells would look in the gram staining procedure if the following mistakes were…
A: Microorganisms are those small organisms that are invisible to the naked eye and can be seen only…
Q: The actual length of a cell is 50µm. When projected on the big screen in the class, the same cell is…
A: Microscopy is one of the basic processes on the basis of which the whole field of Biology rests.…
Q: You can perform a specific staining method to confirm the purity on the culture. Describe this…
A: Microscopic samples are generally stained prior to analysis as it enhances their contrast and aids…
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- The following is a Mannitol Salt Agar plate. What does the yellow color on the right side of this plate indicate? TAW ASM's MicrobeLibrary Ⓒ Reynolds tolerance of the salt mannitol metabolism tolerance of the dyes and chemicals in the agar lactose metabolismIn disc diffusion studies, where will the lowest concentration of the tested chemical be in the agar plate? in the agar directly below the disc O in the agar halfway between the disc and the petri dish wall O in the agar furthest away from the disc the cehmical is equally distributed throughout the agarA culture consisting of 100 litres of nutrient medium containing 12 g l-1 of growth limiting substrate is inoculated with 10g of bacteria in a batch fermenter. The yield of biomass from substrate is 0.05 g g-1 . The maintenance coefficient is 2.0 g g-1 h -1 . The specific rate of product formation due to maintenance is 1.0 h-1 . The maximum specific growth rate of the organism is 0.3 h -1 . YPX= 7.7 and product formation is directly linked with metabolism. a) What is the time required to produce 15 g of biomass? !!!!! ANS IS 3.07 HRS!!!! STEPS on how to reach this please
- If you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock solution would you add to 125 ml of medium? how to calculate these kind of question in tissue culture media preperation1 H 2 176 old culture Smear too BOTERC Losk like? + b. Failure to apply the decolorizer. + ne c. Failure to apply the safranin. Determine whether bacteria are motle or non-motle by looking at the growth in semi-solid medium What is the concentration of agar in (a) typical solid medium (b) semi sold motility medium? Arawer Q1 on Page 357. 7. Orygen Requirement: + d. Reversal of crystal violet and safranin stains. A questions 1.2 and 3 o Pg 83-84 Refer to the power point Questions 1 Predict the effect of the following "mistakes" made when performing a Gram stain. Your answer needs to address the predicted effect on both Gram-positive and Gram-negative cells. Consider each mistake independently. a. Failure to add the iodine. 414 gait to fer. 2 Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram-positive and Gram-negative cells. This being the case, explain how they stain different cell types in the Gram stain. why? a cell wall. patem in…Describe how a Triple Sugar Iron agar tube would appear if the following situation was true. Please note that all items in the following list are occurring in the same tube of agar. 1. Positive for glucose fermentation 2. Negative for lactose and sucrose fermentation 3. Positive for H2S production 4. Negative for gas production
- The following ingredient list represents which type of growth media? Beef extract 1.5 g Yeast extract 3.0 g Peptone 6.0 g Glucose 1.0 g Agar 15.0 g water 1000 ml pH 6.6The principle and What type pf Media of the following Conkey Agar vii) MacConkey Broth viii) TSA Base for Blood and chocolate Agar ix) Mannitol Salt Agar x) Tryptic Soy Agar xi) Alkaline peptone water What is the principle of Growth Medium and Culture medium?Create a graph of this spectrophotometric data (using Excel or the graph paper below), labeling the four stages of the growth curve- lag, log (exponential), stationary, death. Be sure to label your axes properly and give your graph a title Time Culture Sampled % Absorbance 0 min 5% 30min 7% 60min 9% 90min 15% 120min 25% 150min 37% 180min 49% 210min 50% 240min 49% 270min…
- You want to prepare 500 mL of the macronutrient and micronutrient stock solutions which have 100X and 1000X higher concentration, respectively, than the required concentration in the culture mediuma)Approximately 4 % (w/v) sucrose and 0.8 % (w/v) agar are added into the medium.Determine the amount of sucrose and agar (in gram) required in order to prepare 200 mL MS medium. Show your workuse these OD numbers to plot growth curve for E. coli K12- and estimate generation time for this culture. Table 1. Absorbance (O.D.600) measured for growing culture of E. coli K12 (time course) Incubation time O.D.600 Incubation time O.D.600 O min 3 hrs 3 hrs 30 min 4 hrs 4 hrs 30 min 5 hrs 5 hrs 30 min 0.11 1.75 30 min 0.20 1.94 1 hr 1 hr 30 min 2 hrs 2 hrs 30 min 0.27 2.24 0.51 2.48 2.31 2.19 0.65 1.30What nutrients do the following media components contain? • Peptone • Yeast extract • Beef extract Potato infusion • Agar Why should agar media be completely dissolved before they are dispensed in tubes and plates? What are the bases for pegging the temperature at 121°C for 15-30 minutes during moist heat sterilization and 180°C for two (2) hours using dry heat sterilization? 1. Can you sterilize culture media using dry heat sterilization? Why is that so? You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms? Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?