If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is completed? Crystal violet Safranin Decolorizer lodine
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Q: Gram negative bacteria do not retain the stain when washed with alcohol.Comment.
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Q: Gram-staining
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- Which of the following are true about a differential stain. There may be more than one answer! No chemicals are needed. More than one stain is used The acid fast stain is an example The Gram stain is an example.Move the images of bacterial cells into the chart to illustrate the color of gram-positive and gram-negative cells after each step of the Gram stain process.You are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the alcohol. What is the appearance of the bacteria at this point? ○ pink ○ colorless ○ blue ○ purple
- move the images of bacterial cells into the chart to illustrate the color of gram-positive and gram negative cells after each step of Gram stain processIn your own words, explain how gram staining is done and how it works.After performing the gram stain you observe your result as gram negative. To help confirm this result what type of method (besides the gram stain) could you perform? Please explain why, the result you would want to observe and the interpretation of the result.
- You have a patient sample and have done a Gram Stain. When you look on the microscope, this is what you see. Which of the following tests would you use next? 中 МАС O HE • EMB O MSAplease label this in the correct order for gram staining. crystal violet, iodine, alcohol, and safraninWhat is the principle behind the production of two different stain using Gram’s staining method
- List the steps of the gram staining procedure in proper sequence and indicate the color of gram-positive and gram-negative bacteria after each step.When performing a Gram stain on a Gram-negative bacteria, why does the bacteria stain pink? Crystal violet stain binds to iodine and remains in the bacterial cell wall because of the thick peptidoglycan layer. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. The alcohol wash removes crystal violet and iodine from the bacteria. Crystal violet stain binds to iodine and remains in the bacteria cell wall because of the thick peptidoglycan layer AND Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink AND The alcohol wash removes crystal violet and iodine from the bacteria.Imagine that you are Gram staining a mixed culture of both gram-positive and gram-negative bacteria. If you omitted the iodine (mordant) step in the procedure, what colors would the bacteria on your slide appear to be? ► The gram-positive bacteria would be purple, and the gram-negative would be pink All of the bacteria would look purple All of the bacteria would look pink The bacteria would not be stained at all (clear)