Sample No. 1 2 3 4 5 Sample No. Sample Identity 1 2 3 A Table 1. Results of the Qualitative Tests of Unknown Lipid Samples Acrolein Test Hubl's Test Copper acetate Test Greenish-blue color Colorless Colorless No distinct odor Pungent odor No distinct odor No distinct odor Pungent odor Brown solution Light pink color Brown solution Light pink color Light pink color Greenish-blue color Colorless Table 2. Sample Identity of Unknown Lipid Samples 5 Ammonium Molybdate Test No precipitates No precipitates No precipitates No precipitates Yellow precipitates Rationale Liebermann- Burchard Test No color change No color change Bluish-green color No color change No color change
Sample No. 1 2 3 4 5 Sample No. Sample Identity 1 2 3 A Table 1. Results of the Qualitative Tests of Unknown Lipid Samples Acrolein Test Hubl's Test Copper acetate Test Greenish-blue color Colorless Colorless No distinct odor Pungent odor No distinct odor No distinct odor Pungent odor Brown solution Light pink color Brown solution Light pink color Light pink color Greenish-blue color Colorless Table 2. Sample Identity of Unknown Lipid Samples 5 Ammonium Molybdate Test No precipitates No precipitates No precipitates No precipitates Yellow precipitates Rationale Liebermann- Burchard Test No color change No color change Bluish-green color No color change No color change
Chapter10: Sample Preparation And Handling
Section: Chapter Questions
Problem 3.1E
Related questions
Question
Transcribed Image Text:Experimental Procedures
1. Obtain five unknown lipid samples from your laboratory instructor.
2. Place approximately 2 ml of each sample in separate test tubes. Use fresh samples for each
qualitative test.
3. For copper acetate test, add 2 ml of the test reagent to the sample and heat in a water bath for 3-5
minutes. Observe and record the color change.
4. For acrolein test, add 2 scoops of KHSO4 to the sample using a spatula. Heat the samples vigorously
through a Bunsen burner or alcohol lamp for 3-5 minutes. Carefully smell the sample afterwards by
wafting of the vapor to your nose. A sharp irritating odor indicates a positive reaction.
5. For Hubl's test, add 5 drops of the test reagent to the sample and shake the mixture thoroughly for 3
minutes. Observe and record the color change.
6. For ammonium molybdate test, add 2 ml of concentrated nitric acid and 5 drops of ammonium
molybdate reagent to the sample. Heat the mixture for 3-5 minutes in a water bath. Observe for
formation of precipitates.
7. For Liebermann-Burchard test, add 1 ml of glacial acetic acid to the sample followed by 5 drops of
concentrated sulfuric acid. Mix carefully for 3-5 minutes. Observe and record the color change.
8. The samples used are the following: pork lard, oleic acid, canola oil, lecithin, and lauric acid. Match
each sample with the reaction profile that you have obtained.
9. State the reason(s) why you match the sample with a particular reaction profile.
Transcribed Image Text:Sample
No.
1
2
3
4
5
1
Sample No. Sample Identity
2
3
4
Table 1. Results of the Qualitative Tests of Unknown Lipid Samples
Acrolein Test
Hubl's Test
Copper acetate
Test
Greenish-blue color
Colorless
Colorless
No distinct odor
Pungent odor
No distinct odor
No distinct odor
Pungent odor
Brown solution
Light pink color
Brown solution
Light pink color
Light pink color
Table 2. Sample Identity of Unknown Lipid Samples
5
Greenish-blue color
Colorless
Ammonium
Molybdate Test
No precipitates
No precipitates
No precipitates
No precipitates
Yellow precipitates
Rationale
Liebermann-
Burchard Test
No color change
No color change
Bluish-green color
No color change
No color change
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Can you specifically verify which one is canola oil, oleic acid, pork lard, lecithin, and lauric acid? Thank you very much!
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