Answer the following questions (not more than 5 sentences/question). Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test. Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.
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Answer the following questions (not more than 5 sentences/question).
- Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use.
- Explain how calcium ions are confirmed present in a solid salt by using flame test.
- Discuss a method to confirm the presence of a) phenol and b)
ketone in a test compound. - How are proteins confirmed present in a sample? Explain the laboratory process.
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Solved in 5 steps
- Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test. Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.Give a vivid outline of the Standard operating procedure of neutralization test.Prepare 50 plates of Sheep's Blood Agar using 100mm petri dish. Add 10% of blood to the media. (Manufacturer's instructions: Suspend 38 g of blood agar base dehydrated powder in 1 liter of distilled or deionized water.) Pour 25 mL of prepared medium per plate. Express answer to the nearest whole number. How many grams of the dehydrated powder should be weighed? Answer: What is the volume of distilled water that should be used in the preparation? Answer: What is the volume of sheep's blood that should be added to the sterilized and cooled blood agar base? Answer:
- Answer the following questions based on Kirby Bauer / MacFarland standards 1-0.5 McFarland standard solution is ideally used for inoculation of plated for Kirby Bauer test. a)True b)False Principle of Kirby Bauer test is the diffusion of antibiotic agents in to Agar based media. a) True b) FalseDraw/illustrate the chemical reaction involved in Lugol’s test and explain the purpose of this qualitative test?Describe the proper technique for performing reagent strip testing.
- Why is a 1:20 dilution of patient serum, rather than undiluted patient serum, used for the qualitative test?Please explain the theoretical background (positive color reactions and what is being detected) involved in the different qualitative test for proteins that has been isolated intact or hydrolyzed. Please directly answer the theoretical background (positive color reactions and what is being detected) of each test GENERAL: BIURET - NINHYDRIN SPECIFIC XANTHOPROTEIC TEST - MILLON'S TEST - HOPKINS-COLE TEST - SAKAGUCHI TEST - LEAD ACETATE TEST - NITROPRUSSIDE TEST - FOLLIN'S REACTION - SULLIVAN'S TEST - PAULY REACTION - LIEBERMAN'S TEST -Paraphrase the text below: Series of test tubes were filled with the desired volume of the BSA (0.1, 0.2, 0.3… 1 ml) .PBS was added to this to make the volume of 1 mL. 5 mL of copper reagent was added to all the tubes. After proper mixing, all the tubes were incubated at room temperature for 15 minutes. 1 mL Folin reagent was added to each and mixed properly with the help of vortex mixer and incubated for 20 minutes. The intensity of the colour was then determined spectrophotometrically at 680 nm. The graph was than plotted between optical density and the amount of BSA. Proteins estimation was done for the detection for the amount of proteins present in the sample solutions by the Lowry method
- The table below summarizes the results for Millon's test. Provide the correct remarks from the results if Negative for Millon's Test or Positive for Millon's Test and if Contains phenolic hydroxy group or Does not contains phenolic hydroxy group.Create a flow chart to perform the required tests to identify unknown microorganism: - Use at least 3 other biochemical tests from the following list: Mannitol salt agar, Blood agar, Starch agar, Tributyrin Agar, Gelatin, Casein agar, Indole Production, MR-VP, Citrate, Hydrogen Sulfide test, Urease test, Nitrate reduction test, Catalase test, and Oxidase test. The Gram positive organisms that could be present in your unknown mixture are: Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis and Lactococcus lactis.Write down the materials, reagents, Principle, procedure and results for Methamine silver technique.