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Q: Hyrolysis of DNA Test Results 1. Inorganic Phosphate 2. Purines 3. Deoxyribose
A: Hydrolysis of DNA will cause the DNA backbone to break down and ultimately give the deoxyribose…
Q: Plasmid map of pUCT8/T9 Eco01091 2674 Pfol 46 Aatil 2617 Sspl 2501 Pdml 2294. Bogl 2216 2486 146/
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Q: Holoenzyme binds the DNA without the assistance of proteins such as TFs. True False
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Q: which sample shows the plasmid is fully digested
A: 50 bp ladder sequence : 500 bp 450 bp 400 bp 350 bp 300 bp 250 bp 200 bp 150 bp 100 bp 50 bp
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A:
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Q: Tailed primer can have restriction enzyme sites. True False
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Q: Which of the following methods for determining protein concentration is most commonly used? O Lowry…
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Q: Polymerase chain reaction cannot amplify complete circular plasmids. True False
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- 16. Your Excel data graph for Serial Dilution lab:** 16a. Include a clear and labeled graph of DF (x axis) versus Absorbance (y axis). See box above for expectations and review video tutorial of this Excel work. Do not graph two of your data points: your blank or your Cuvette #1 (DF=1). 1 Q A Multiple Styles W S X 3 E D $ 4 To C R F % 5 I T V U ^ 6 G Y 7 H U 8 J BN S ( 9 K M X[protein] volume [protein] [protein] in to Sample Lysate in "gel- load 5 in 20 protein in Name volume assay* lysate ready" ul sample protein 3.9 2 µl Hg/ml Tilapia 19.9 10 μι Hg/ml 18.2 2 µl Hg/ml Cod >20 14 10 μι Hg/ml 9.4 15 2 µl ug/ml Sole >20 6. 10 μι ug/ml > >Normal text Calibri BI UA G |EE| +2 I +3+1 CICCO4, 1 LE | ZJ VITICHI SUpstances did not test positive for any of the organic compound Distilled water did not test positive for any of the organic compounds. 5| 6 3) 4) What do all of the indicators you have used have in common? Indicators are all substances whose solutions change color due to changes in p 5) Is there a macromolecule that the tests in this lab did not test for No, there is no macromolecule that the lab did not test for. 6) Which compound is most common in foods that come from plants? Cellocuse is the single most common biochemical compound in living things. 7) If you wanted to reduce the amount of fat in your diet, what foods would you avoid? Steer away from foods high in saturated and trans fats like fried foods, fatty meats, stick margarine, snacks and items with hydrogenated oil. M 6 8) Which foods tested would your body use for a quick burst of energy? a. For energy when no carbohydrates are available? b. For building…
- o NB4: Why do you think you first had to make the 1 mM solution instead of working directly from the stock solution?The order is for Moxam 5 gm IM every 8 hours. On hand is 10 gram of Moxam with the following directions. Add 8 mL of diluent to yield 10mL of reconstituted solution. How many mL will you give?AE, 62, Male Wt: 75 kg, Ht: 150 cm Orders: Penicillin G Potassium 10,000,000 Units Sterile water for injection qs Make a 250,000 U/mL solution. The package insert states If Penicillin G Potassium 10,000,000 Units is reconstituted with 44 mL sterile water, a solution of 200,000 U per mL will be obtained. What volume of sterile water would you use to reconstitute the penicillin G potassium in order to make the ordered solution?
- The SDS-PAGE protocol requires 0.5L of running buffer for the gel apparatus. The stock running buffer comes as a 20x concentrate. How should you prepare your running buffer? 50ml 20x stock + 450ml deionized water 450ml 20x stock + 50ml deionized water O 250ml 20x stock + 250ml deionized water 25ml 20x stock + 500ml deionized water 25ml 20x stock + 475ml deionized water 2ml 20x stock + 500ml deionized water O 100ml 20x stock + 400ml deionized waterSketch the appearance after visualization of a protein mixture containing the seven proteins (fibrinogen, y-globulin, collagen, ovalbumin, myoglobin, hemoglobin, insulin) when subjected to two-dimensional (2D) gel electrophoresis.Figure 3: ● ● ● ● ● ● KDa ● 97.4 66.2 45.0 ● 31.0- 21.5 14.4 S-1 p-1 S-2 2-0 This figure was generated by centrifuging a pura sample of protein, removing the supernatant, and resuspending the pellet in the same volume as the supernatant to allow direct comparison. The supernatant and pellet samples were then prepared for SDS-PAGE identically and run via normal SDS-PAGE procedures. In the figure, "s" means supernatant and "p" means pellet. The text or number after the dash represents a different condition. For example, s-1 and p-1 are the supernatant and pellet samples under condition 1. It is not shown, but under wild-type conditions, essentially all of the protein is found in the supernatant. S-3 ● What does the intensity of each band represent? ● Would you find soluble protein in the supernatant or pellet? Why? Would you find aggregated protein in the supernatant or pellet? Why? For each condition (there are 5 different conditions), is there a higher percentage of the total protein…
- For the following sequence, what is the approximate annealing temperature? 5'-AGCTACGATCAGGTCA-3'Large proteins travel/migrate faster in both gel filtration chromatography and SDS- PAGE. O True O False50 mg/50 ml kinetin stock is available You need 2.5 mg/l kn for Ms media calculate amount of kn is required? Explain the role of surface sterilising chemicals in aseptic culture initiation and function of sucrose in MS medium?