1 2 3 4 5 6 N Sample Lysate Name volume 8 Tilapia Cod Sole Perch Tilapia Cod Sole 2 μl Perch 10 με 2 μ. 10 με 2 μl 10 μl [protein] [protein] in in assay lysate 3.9 µg/ml 19.9 µg/ml 18.2 µg/ml > 20 µg/ml 9.4 µg/ml 2 μι 10 με *assay range: 2-20 µg/ml What volume of the each fish "gel-ready" sample should you load on your gel to load 5µg of protein? > 20 µg/ml <2 µg/ml 7.3 µg/ml [protein] volume in to load 5 "gel- ready" μg sample protein 7.3 μl 4.2 μl μg protein in 20 μl 19.5 μl 4.2 μl > <
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