50 mg/50 ml kinetin stock is available You need 2.5 mg/l kn for Ms media calculate amount of kn is required? Explain the role of surface sterilising chemicals in aseptic culture initiation and function of sucrose in MS medium?
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- Time point (min) Absorbance of culture at 660nm Approximate cell concentration Approximate # cells in 1mL extract 0 0.298 1.49 x 108 cells/mL 1.49 x 108 cells 10 0.316 1.58 x 108 cells/mL 1.58 x 108 cells 20 0.374 1.87 x 108 cells/mL 1.87 x 108 cells 30 0.429 2.145 x 108 cells/mL 2.145 x 108 cells 40 0.512 2.56 x 108 cells/mL 2.56 x 108 cells 50 0.544 2.72 x 108 cells/mL 2.72 x 108 cells 60 0.607 3.035 x 108 cells/mL 3.035 x 108 cells a. Using these data, prepare a growth curve of this strain ofEscherichia coli (E. coli).b. Estimate the doubling time for this strain of E. Coli. Clearly showhow you estimated this value from the empirical data presented.Inoculate 250-µL overnight cell culture into 50 ml LB medium (in a 250 ml flask). Shake vigorously at 37 °C to OD600~0.5-0.6 (usually it takes about 2-3 hours). Why should we use a larger flask during culture at this step? Why do we need to wait till this OD range is achieved?Prepare the following LB media with antibiotic added. A 100ml of LB media with 25 micro/ml of Amp and 100micro/ml of Kan final concentration. A 100ml of LB is provided and amp of Kan stock at 100 mg/ml and 50 mg/ml are also provided. Find how much each antibiotic stock solution is needed to add 100ml of LB to reach desired antibiotic concentration.
- What is the result of this biochemical test in the gelatin deep?cWhich enzyme is this testing for? What tool is used to inoculate this medium?If you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock solution would you add to 125 ml of medium? how to calculate these kind of question in tissue culture media preperationFollowing electrophoresis of the two pGlo mini-preparation samples (both digested and undigested), the following agarose gel image was obtained. provide an accurate and detailed figure, describing the image and what it depicts.
- Which stationary phase is better in separation of the components of moringa extract for column chromatography? Sodium Bicarbonate or Silica Gel? And Explain.Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)1. What are the functions of the sample buffer and sample reducing agent? Why do the samples need to be heated before you load them on the gel?2. What determines the current in your gel? What could cause the current in your gel unit to be lower than expected? Is there anything that could cause it to be higher than expected?3. Why do you need to wash the gel before staining it? Why use warm water?Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) 1. What determines the current in your gel? What could cause the current in your gel unit to be lower than expected? Is there anything that could cause it to be higher than expected? 2. Why do you need to wash the gel before staining it? Why use warm water?
- Determination of MW by Gel electrophoresis100ml of LB media with 25 μg/ml of Amp and 100 μg/ml of Kan final concentration. You have 100ml of LB provided and Amp and Kan stocks at 100 mg/ml and 50 mg/ml provided. Determine how much of each antibiotic stock solution you need to add to 100ml of LB to reach desired antibiotic concentration.Relative fluorescence at 603 nm 1.0 0.8 0.6 0.4 0.2 0.0 L 0.00 0.10 0.20 0.30 Time (s) 0.40 0.50 0.60 In this FRET data table, an antibiotic was added, but the type was not recorded. According to the results, could the buffer being used be either negamycin, tetracycline, rifamycin, or cycloheximide? Explain why based on the FRET data.